摘要
目的:建立一步法荧光定量RT-PCR方法快速检测肠道病毒EV71。方法:从Genebank中找出最近2-3年中国大陆区域流行的EV71病毒VP1基因的序列进行比对,找出2-3个相对保守区域设计引物及taqman探针。筛选出一套表现良好的引物并进行反应条件的优化。结果:筛选出一套对EV71病毒具有高度灵敏度和特异性的引物及taqman探针,检测灵敏度达10copiesVP1/μL,整个操作过程仅需2-3h。结论:该套引物及探针针对EV71具有良好特性,是用于EV71的日常监测和暴发时的应急诊断。
Objective：To establish an one - step real time RT - PCR technology for quickly detection EV71 virus. Methods：The sequences of VPI gene in EV71 viruses which are recently prevalent in mainland of China were downloaded and compared. Then 2 or 3 comparative conserve regions were selected as the target for real time RT - PCR. Results ： The best primers and probe suit of designed primers and probe sets was selected by experiments. The method has high specificity and sensitivity, the detection limit is 10 copies VP1/μL. The detection experiments will cost only 2 -3h. Conclusion：The method has high quality of detection EV71 virus. It can be used as the technology for dally inspection or the emergence method in prevalence of EV71.
出处
《医学信息（中旬刊）》
2011年第5期1896-1897,共2页
Medical Information Operations Sciences Fascicule
