高效价麻疹抗血清的制备及检定

Preparation and Quality Control of High Titer Antiserum against Measles Virus

目的制备高效价麻疹抗血清,用于疫苗检定中病毒鉴别、支原体检测、麻腮二联和麻腮风三联疫苗的联合滴定试验。方法将麻疹病毒L4株于Vero细胞中传代培养,获得高滴度病毒原液,制备免疫抗原,分别经腹腔注射法、耳静脉注射法和背部皮下多点注射法免疫家兔,制备抗血清,并按规程要求对抗血清进行检定。结果采用腹腔注射法、耳静脉注射法和背部皮下多点注射法免疫家兔所制备的抗血清的中和效价分别为1∶960、1∶2 560和1∶3 840,经检定,3种方法制备的抗血清均达到疫苗检定的使用要求。结论背部皮下多点注射法制备的麻疹抗血清效价最高,且操作简单,可作为制备高效价麻疹抗血清的最佳方案。

Objective To prepare high titer antiserum against measles virus and use for the virus identification,mycoplasma examination in control tests on vaccine as well as combined titration test on MM and MMR vaccines.Methods Measles virus L4 strain was subcultured in Vero cells to obtain high titer virus bulk and prepare the antigen for immunization.Rabbits were injected with the prepared antigen by i.p.,i.v.and s.c.routes respectively,and the obtained antisera were subjected to control tests according to the requirements in Chinese Pharmacopoeia.Results The neutralizing titers of antisera prepared by immunization of rabbits by i.p.,i.v.and s.c.routes were 1 ∶ 960,1 ∶ 2 560 and 1 ∶ 3 840 respectively.All the antisera met the requirements for control tests on vaccine.Conclusion The preparation of antiserum against measles virus by s.c.injection in several sites on back of rabbits was simple,and the prepared serum reached the highest titer,which might be an optimal method for preparation of high titer antiserum against measles virus.