RhoA和核因子κB在胃癌中的表达及其临床意义

Expression and significance of RhoA and NF-KB in human gastric carcinoma

目的探讨RhoA和核因子KB（NF-KB）在胃癌组织中的表达，分析其与胃癌患者临床病理特征及预后的关系。方法利用免疫组化、组织芯片技术检测189例胃癌、54例相应癌旁及32例正常胃黏膜组织中RhoA蛋白和NF-KB蛋白的表达。用Kaplan-Meier法行单因素生存分析，应用Cox回归模型进行多因素生存分析。结果RhoA蛋白在胃癌、癌旁和正常胃黏膜组织中的表达阳性率分别是84．7％、68．5％和65．6％，胃癌与癌旁及正常胃黏膜组织的阳性率差异有统计学意义（P〈0．05）。NF-KB蛋白在胃癌、癌旁和正常胃黏膜组织中的表达阳性率分别是75．1％、42．6％和15．6％，且三者的阳性率相互比较，差异均有统计学意义（P〈0．05）。RhoA和NF-KB在胃癌组织中的蛋白表达呈正相关（r=0．203，P=0．005）。RhoA的蛋白表达与胃癌的浸润深度有关（P〈0．05）。NF．KB的蛋白表达与胃癌的浸润深度和有无淋巴结转移有关（P〈0．05）。单因素分析结果显示，肿瘤大小、有无淋巴结转移、浸润深度和NF-KB的蛋白表达影响胃癌患者术后的生存（P〈0．05）；多因素回归分析结果显示，NF-KB蛋白表达、有无淋巴结转移和浸润深度为影响胃癌患者预后的独立因素（均P〈0．05）。结论RhoA和NF-KB参与胃癌的发生、发展，并在胃癌的浸润和转移中起重要作用；NF-KB的蛋白表达水平、有无淋巴结转移和浸润深度是影响胃癌患者预后的独立因素。

Objective To investigate the expression of RhoA and NF-KB in gastric carcinoma and their correlation with clinicopathological fearures. To determine the effective prognostic factors of long-term suivival of gastric carcinoma patients. Methods The role of RhoA and NF-KB in gastric carcinoma was assessed by tissue array technology and the levels of RhoA and NF-KB expression in paraffin-embedded tissues was quantified by immunohistochemistry from 189 cases of gastric carcinoma, 54 cases of their adjacent tissues, and 32 cases of normal gastric mucosa. The prognosis of gastric carcinoma was evaluated by Kaplan-Meier survival analysis and Cox multivariate regression analysis. Results The positive rates of RhoA expression were 84.7%, 68.5% and 65.6% in gastric carcinoma, adjacent tissues and normal mucosa, respectively. The expression of RhoA in gasric carcinoma was significantly higher than that in adjacent tissues and normal mucosa （P 〈 0.05 ）. The positive rates of NF-KB expression were 75.1%, 42.6% and 15. 6%% in gastric carcinoma, adjacent tissues and normal mucosa, respectively. The expression of NF-KB in gasric carcinoma was significantly higher than that in adjacent tissues and normal mucosa （ P 〈 0. 05 ）. RhoA was positively linked with NF-KB （ r = 0. 203, P = 0. 005 ）. In gastric carcinoma, the expression of RhoA was related with depth of invasion （ P 〈 0.05）, and the expression of NF-KB was related with depth of invasion and lymph node metastasis （ P 〈 0. 05 ）. The Kaplan-Meier survival analysis showed that the tumor size, lymph node metastasis, depth of invasion, expression of RhoA and NF-KB can shorten the cumulative survival rate. With these paramaters entering the Cox multivariate regression analysis mode, it was revealed that expression of NF-KB, lymph node metastasis and depth of invasion are independent prognostic factors. Conclusions The overexpression of RhoA and NF-KB isinvolved in the occurrence and development of gastric carcinoma. RhoA is positively linked with NF-KB. They are correlated with the invasion and metastasis of gastric carcinoma. The expression of NF-KB, lymph node metastasis, depth of invasion are independent prognostic factors playing an important role in prediction of the clinical outcome after radical resection of gastric carcinoma.