溃疡性结肠炎患者血清白细胞介素8和肿瘤坏死因子-α的水平和意义

Clinical significance of serum IL-8 and TNF-α in patients with ulcerative colitis

目的 探讨白细胞介素8（IL-8）和肿瘤坏死因子-α（TNF-α）在溃疡性结肠炎（UC）发病中的作用及临床意义.方法 采用酶联免疫吸附法（ELISA）测定47例活动期UC患者（其中轻度15例、中度18例和重度14例）,21例缓解期UC患者及20例健康对照组的血清IL-8和TNF-α水平.结果 活动期UC患者血清IL-8和TNF-α水平分别为（58.3±11.6）、（62.1±10.8）ng/L,显著高于缓解期UC患者和健康对照组（均P＜0.05）,缓解期UC患者IL-8和TNF-α水平显著高于健康对照组（均P＜0.05）.重度活动期UC血清IL-8和TNF-α水平高于中度和轻度活动期UC,中度活动期UC血清IL-8和TNF-α水平高于轻度活动期UC,组间比较均差异有统计学意义（P＜0.05）.结论 IL-8和TNF-α参与了UC的炎性过程,检测血浆IL-8和TNF-α可作为判断UC患者病变严重程度和活动性的重要指标.

Objective To evaluate the clinical significance of serum IL-8 and TNF-α in patients with ulcerative colitis. Methods Enzyme-linked immunosorbent assay（ELISA） was used to detect the level of serum IL-8 and TNF-α in47 patiens wih active phase UC（severe stage 14 cases,medium stage 18 cases,mild stage 15 cases） ,21 patients with catabasis UC and 20 healthy individuals. Results The levels of IL-8 and TNF-α in patients with active phage UC were significantly higher than those of patients with catabasis UC and healthy individuals（P ＜ 0.05）. The levels of IL-8 and TNF-α in patients with catabasis UC were higher than healthy individuals（P ＜0.05）. The levels of IL-8 and TNF-α in severe UC were higher than those of patients with medium UC and mild UC （P ＜ 0.05）. The levels of IL-8 and TNF-α in medium UC were higher than those of patients with mild UC （P ＜ 0.05）. Conclusion The IL-8 and TNF-α may play an important role in the pathogenesis of UC. The combined levelsof serum IL-8 and TNF-α were potential markers in the evaluation of the activity and seriousness of UC.