改进血清铜蓝蛋白凝胶电泳—血清铜蓝蛋白氧化酶活性的邻联茴香胺显示法

Improved gel electrophoresis of ceruloplasmin detecting ceruloplasmin oxidase activity of human serum with O-Tolidine

目的：介绍一种改进分离血清铜蓝蛋白的方法。方法：利用不连续缓冲体系琼脂糖—淀粉凝胶电泳进行分离，再利用血清铜蓝蛋白催化邻联茴香胺基质来显示酶活性区带。结果：该方法方便，显色稳定，分离效果好。结论：通过对一个Ｗｉｌｓｏｎ 病家系分析，说明本法有重要的临床价值。

Starch gel electrophoretic method was improved to separate ceruloplasmin.Agarose-starch mixed gel was used with discontinuous buffer system.Its principle is based on serum ceruloplasmin catalyzing O-Tolidine to coloured band.This method is convenient and stable and proved its clinical value by analyzing a family of wilson's diease. [