摘要
目的 探讨降钙素基因相关肽（ＣＧＲＰ）在蛛网膜下腔出血（ＳＡＨ）后脑血管痉挛（ＣＶＳ）发生发展中的作用机制。方法 采用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）技术检测大鼠ＳＡＨ 后ＣＶＳ脑组织、血管ＣＧＲＰ ｍ ＲＮＡ 表达。结果 正常对照组ＣＧＲＰｍ ＲＮＡ 表达水平依次为基底节区、海马、大脑皮层和脑血管；注血后３ｄ 各脑区、血管ＣＧＲＰｍ ＲＮＡ 表达均较正常对照组、注血后３０ｍ ｉｎ、注血后７ｄ 显著下调（Ｐ＜ ０．０１）。结论 ＣＧＲＰ ｍ ＲＮＡ 表达下调、舒缩血管物质失衡是ＳＡＨ 后ＣＶＳ的主要原因之一。
Objective To explore mechanism of CGRP on CVS following SAH. Method The expressions of CGRP mRNA in rat brain and blood vessel on CVS following SAH were used by RT PCR technology. Results Expression levels of CGRP mRNA were successively basal nuclei,hippocampus,cerebral cortex and blood vessel in control group. Expression levels of CGRP mRNA in this cerebral area and blood vessel at third day after injecting blood were lower than that one in control,30min group 7th day group after injecting blood (P<0.01 respectively). Conclusion Decreasing levels of CGRP mRNA expression and disorder of vasoconstrictive and vasodilative substance are an important reason to induce CVS following SAH.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
1999年第5期260-262,共3页
Journal of Apoplexy and Nervous Diseases
