戊四氮致痫对大鼠海马星形胶质细胞的影响

Effect of epileptic seizure induced by PTZ on the hippocampal astrocytes in rats

目的 探讨戊四氮（ＰＴＺ）致痫对大鼠海马星形胶质细胞的影响。方法 应用免疫组织化学方法观察ＰＴＺ致痫后大鼠海马内胶原纤维酸性蛋白（ＧＦＡＰ）变化的特点，同时观察了不同强度的痫性发作与ＧＦＡＰ改变的关系。结果 ＧＦＡＰ改变于ＰＴＺ致痫后１２ｈ 开始，２４ｈ 达到高峰，７２ｈ 已开始回落，并且痫性发作的强度与ＧＦＡＰ改变有一定的联系。结论 癫痫与星形细胞之间确有一定联系。

Objective To explore the effect of epileptic seizure induced by pentylenetetrazol (PTZ) on the hippocampal astrocytes. Methods The time charactery of glial fibrillary acidic protein (GFAP) in hippocampus was observed in rats following administration with PTZ,via immunocytochemistry. Simultaneously,the relationship between seizure degree and GFAP was observed too. Results After the rats were administered with PTZ,GFAP change began at 12h,reached peak at 24h and began its reduction at 72h,and GFAP changes were associated with seizure degrees. Conclusion Epileptic seizure is precisely associated with astrocyte changes.