Annexin A5表达与鼻咽癌分化转移的关系

Relationship between Expression of Annexin A5 and Differentiation and Metastasis of Nasopharyngeal Carcinoma

目的探讨Annexin A5表达与鼻咽癌分化和转移的关系。方法采用免疫组织化学方法检测65例鼻咽癌、18例淋巴结转移性鼻咽癌和20例慢性鼻炎标本中Annexin A5的表达水平,并分析其表达水平与鼻咽癌临床病理特征的关系;采用Western blot检测不同分化程度和不同转移潜能NPC细胞系中Annexin A5的表达;采用siRNA技术下调5-8F细胞中Annexin A5表达后,观察其侵袭能力的变化。结果 Annexin A5的阳性表达率在鼻咽癌、淋巴结转移鼻咽癌和对照组中分别为84.6%(55/65)、94.4%(17/18)和10.0%(2/20);淋巴结转移性鼻咽癌组高于鼻咽癌组,鼻咽癌组高于对照组,差异有统计学意义(P<0.05)。Annexin A5表达与组织学类型、局部转移、淋巴结转移、远处转移、临床分期和复发有关(P<0.05),而与年龄,性别无关。Annexin A5表达下调能降低NPC细胞的侵袭能力。结论 Annexin A5表达与鼻咽癌的分化和转移有关,可作为NPC分化、转移及预后的分子标志物。

Objective To study the relationship between expression of Annexin A5 and the differentiation and metastasis of nasopharyngeal carcinoma（NPC）.Methods The expression of Annexin A5 in specimens of 65 of NPC,18 of lymph node metastasizing NPC（LMNPC） and 20 of chronic rhinitis（CR） were detected by immunohistochemistry.The correlation between the expression of Annexin A5 and clinic factors was analyzed.The expression of Annexin A5 in NPC cell lines with differential differentiation degree and metastasis potency was detected by Western blot.Observe the change of invasive capability of NPC cells after the downregulation of Annexin A5 by siRNA.Results The positive ratio of Annexin A5 in NPC,LMNPC and CR were 84.6%（55/65）,94.4%（17/18） and 10.0%（2/20）,respectively.There were significant difference in the expression of Annexin A5 between NPC and LMNPC,NPC and CR（P0.05）.There were correlation among the expression of Annexin A5 and histological type,regional meastasis,distant metastasis,clinic stage and recurrence（P0.05）.And was no relationship with ages and gender.There was relationship among expression of Annexin A5 and differentiation and metastasis of NPC.Downregulation of Annexin A5 can decrease the invasive capability of 5-8F cells.Conclusion There was relationship among the expression of Annexin A5 and the differentiation and metastasis of NPC,and could serve as a biomarker of differentiation,metastasis and prognosis in NPC.