HGF、c-Met和VEGF-C在宫颈癌中的表达

Expression of HGF,c-Met and VEGF-C in Cervical Carcinoma

目的检测宫颈癌组织中HGF及其受体原癌基因c-Met和VEGF-C的分子表达水平及三者间的关系。方法采用实时荧光定量RT-PCR(Real Time fluorescence Quantitative PCR,FQ RT-PCR)技术相对定量检测43例宫颈浸润癌,30例CINⅢ和27例正常宫颈组织标本中HGF mRNA、c-Met mRNA和VEGF-C mRNA的表达水平。结果 HGF mRNA、c-Met mRNA和VEGF-C mR-NA在正常宫颈、CINⅢ和宫颈浸润癌组织中的表达水平依次升高,差异有统计学意义(P<0.05)。宫颈癌组织中三者表达水平与肿瘤临床分期、肿瘤直径大小、浸润深度、淋巴结转移有关(P均<0.05)。HGF mRNA与c-Met mRNA和VEGF-C mRNA表达相关(P<0.01)。结论 HGF、c-Met和VEGF-C在宫颈癌发生、发展、转移中发挥重要作用,它们参与了宫颈癌新生血管和淋巴管的形成,对宫颈癌的诊断、治疗及预后有指导意义。

Objective To investigate the expression level of hepatocyte growth factor（HGF） and its receptor,the proto-oncogene c-Met,and vascular endothelial growth factor-C（VEGF-C） in cervical carcinoma.Methods The mRNA levels of HGF,c-Met and VEGF-C were detected by Real Time fluorescence Quantitative PCR（FQ RT-PCR） method in 43 cervical carcinoma tissues,30 cervical intraepithelial neoplasm Ⅲ（CINⅢ）tissues and 27 normal cervical,respectively.Results The positive expression of HGF mRNA,c-Met mRNA and VEGF-C mRNA increased remarkably from normal tissue to CINⅢ and then to cervical carcinoma（P0.05）.In cervical carcinoma,the expression of these three genes was positive correlated with the clinical stage,tumor size,the invasion of deep muscular layer and the lymph node metastasis（P0.05）.These three genes were significant positive correlation（P0.01）.Conclusion Over expression of HGF,c-Met and VEGF-C together may participate in the invasion and metastasis of cervical carcinoma.To investigate them together would be facilitated to the treatment and prognosis of cervical carcinoma.