荧光原位杂交在子宫颈病变的癌变风险预测中的应用

Application of Fluorescence in Situ Hybridisation into Forecasting Canceration Risk of Cervix Disease

目的探讨hTERC基因在不同宫颈病变中的扩增情况及hTERC基因扩增对宫颈病变发展趋势的预测意义。方法利用FISH在荧光显微镜下观察3号染色体着丝粒CSP3和hTERC基因双色探针的杂交效果,检测不同宫颈病变中hTERC基因扩增情况,对部分存在hTERC基因扩增的患者进行随访。结果随着宫颈病变的发展,hTERC基因扩增的阳性率及扩增的细胞所占比例均在不断升高,差异具有统计学意义。结论 hTERC基因扩增与宫颈病变的发展密切相关,FISH检测hTERC基因是否存在扩增可能成为预测宫颈病变发展趋势的可靠手段。

Objective To explore the circumstance of hTERC gene amplification in the differ cervix disease and the forecasting significance between hTERC gene amplification and developmental tendency of cervix.Methods Using fluorescence microscope to observe the crossing effection of centromeric probe for 3 chromosome and the probe of hTERC gene.The circumstance of hTERC gene amplification is detected in the differ cervix disease.Meanwhile,the cases which have hTERC gene amplification are followed up.Results Amplification of hTERC gene is enhanced along with the development of cervix disease which has statistical significance.Conclusion There is close correlation between the hTERC gene amplification and the development of cervix disease,Using FISH to detect hTERC gene amplification may be the dependable means to forecast the developmental tendency of cervix disease.