异丙酚对大鼠失血性休克复苏后肠道的保护作用

Protective Effect of Propofol on Intestine after Hemorrhagic Shock and Resuscitation

目的:探讨短时间内异丙酚对失血性休克复苏后肠道的保护作用。方法:将大鼠随机分为假手术组(C组)、失血性休克复苏组(HS-R组)和异丙酚组(P组),每组8只。建立失血性休克复苏动物模型,HS-R组、P组分别在复苏后采用微量泵持续泵入生理盐水1mL·kg-1·h-1、异丙酚1mL·kg-1·h-1,维持2h,测血浆中二胺氧化酶(DAO)水平;开腹取小肠组织约5cm,3cm匀浆后分别采用黄嘌呤氧化酶法、硫代巴比妥酸比色法测超氧化物歧化酶(SOD)和丙二醛(MDA)水平,2cm做病理组织切片HE染色,观察肠道组织病理学改变。结果:HS-R组及P组的MDA及DAO水平均较C组升高,P组较HS-R组有所降低(均P<0.05);HS-R组及P组的SOD水平较C组降低,P组较HS-R组有所升高(均P<0.05);肠道组织病理学评分P组及HS-R组均高于C组,但P组较HS-R组低(均P<0.05)。结论:失血性休克复苏后肠道组织出现明显的损伤,短时间内异丙酚可通过抗氧自由基作用减轻此损伤。

Objective： To determine the protective effect of propofol on intestine after hemorrhagic shock and resuscitation in rats when used for a short period of time. Methods： Thirty Wistar rats were randomized into 3 groups,sham operation group （C group）, resuscitation group （HS-R group） and propofol group （P group）. There were 8 rats in each group. The animal model of hemorrhagic shock and resuscitation was established. Following resuscitation, the rats of HS-R group were infused normal saline at 1 mL·kg-1·h-1 with infusion pump for 2 hours, and rats of P group were infused propofol at 1 mL·kg-1·h-1 for 2 hours. The level of blood diamine oxidase （DAO） was measured in rats of groups. The intestinal tissue of 5 cm was taken. The levels of superoxide dismutase （SOD） and malondialdehyde （MDA） were measured using 3 cm of the tissue by xanthinoxidase method and thiobarbituric acid colorimetric technique. The other 2 cm of intestinal tissue was used to do HE staining to observe the changes of intestinal pathological histology. Results： The levels of MDA and DAO were higher in HS-R group and P group than those in C group （P 0.05）,and were lower in P group than those of HS-R group（P 0.05）. The level of SOD was lower in HS-R and P groups than that of C group （P 0.05）, and it was higher in P group than that of HS-R group （P 0.05）. The pathological grades of intestine were higher in P group and HS-R group than those in C group （P 0.05）, but were lower in P group than those of HS-R group （P 0.05）. Conclusion： The intestinal tissue was significantly damaged after hemorrhagic shock and resuscitation.Propofol can reduce this damage by against the oxygen free radicals in short-term.