十溴联苯醚（BDE-209）染毒对雄性BALB/c小鼠睾丸毒性作用

Studying the lipid peroxidation index, morphology and apoptosis in testis of male BALB/c mice exposed to polybrominated diphenyl ether （BDE-209）

目的 探讨十溴联苯醚（BDE-209）染毒对雄性BALB/c小鼠睾丸组织脂质过氧化指标的影响及其睾丸组织形态学变化.方法 21只雄性BALB/c小鼠随机分为高剂量染毒组、低剂量染毒组和对照组,每组7只,分别给予500 mg/kg BDE-209（高剂量组）、200 me/kg BDE-209（低剂量组）和0.1ml/10 g体重生理盐水（对照组）,经灌胃给药,每日1次,连续染毒6周.测定小鼠体重、睾丸重量,测定睾丸组织还原型谷胱甘肽（GSH）、丙二醛（MDA）含量和超氧化物歧化酶（SOD）活力,观察睾丸组织形态学变化,TUNEL法检测睾丸细胞凋亡的改变.结果 高、低剂量组小鼠体重和睾丸重量均明显低于对照组,差异有统计学意义（P＜0.05）.高剂量染毒组睾丸脏器系数为（0.8640%±0.1706%）明显高于对照组（0.8329%±0.1386%）,差异有统计学意义（P＜0.05）.高、低剂量染毒组睾丸组织中GSH水平和SOD活力分别为（0.044±0.006）、（0.039±0.005）nmol/mg和（0.735±0.179）、（0.907±0.198）U/mg,明显低于对照组[（0.052±0.067）nmol/mg prot]和[（1.161±0.188）U/mg],差异有统计学意义（P＜0.05）;高、低剂量染毒组睾丸组织MDA含量分别为（2.365±0.339）、（1.752±0.366）nmol/mg,高于对照组[（1.173±0.232）nmol/mg],差异有统计学意义（P＜0.05）.与低剂量染毒组相比,高剂量染毒组睾丸组织中SOD活力降低,MDA含量升高,差异有统计学意义（P＜0.05）.组织形态学观察可见,染毒组生精细胞的数目和层次明显减少,且排列层次紊乱,支持细胞数目减少,小管中心明显萎缩.TUNEL实验结果表明,染毒组睾丸细胞出现少量凋亡细胞.结论 BDE-209可引起雄性BALB/c小鼠睾丸组织脂质过氧化指标的改变,对睾丸有一定的毒性作用.

Objective To explore the lipid peroxidation and the testicular morphological change induced by decabrominated diphenyl ether （BDE-209） in male BALB/c mice. Methods Twenty one male BALB/c mice were randomly divided into three groups： the high exposure group （500 mg/kg BDE-209）, the low exposure group （200 mg/kg BDE-20） and control group （normal saline）. The mice were exposed by gavage one time a day for 6weeks, then were sacrificed. Body weight, testis weight, malonyldialdehyde （MDA）,total supemxide dismutase （T-SOD） and glutathione （GSH） in testis were examined. the morphological alteration of testis was observed. TUNEL assay was used to detect the apoptosis in testicular cells. Results Body weight and testis weight in high and low exposure groups were （21.6140 ± 2.3550）g, （20.8000 ±1.7630）g and （0.1859±0.0349） g, （0.1718±0.0266） g, respectively, which were significantly lower than those （27.7570±1.2880） g and （0.2302±0.0335）g in the control group （P＜0.05）; the testis coefficient in high exposure group was （0.8640%±0.1706%）, which was significantly higher than that （0.8329±0. 1386%） in the control group （P＜0.05）. The GSH level and SOD activities of testis in 2 BDE-209 groups were 0.044±0.006,0.039±0.005 nmol/mg prot, and 0.735±0.179, 0.907±0.198 U/mg prot, respectively, which were significantly lower than those （0.052±0.067） mol/mg and （1.161 ±0. 188） U/mg in the control group （P＜0.05）. The levels of MDA in 2 BDE-209 groups were （2.365±0.339） and （1.752±0.366） nmol/mg prot, which were significantly higher than that （1.173±0.232 nmol/mg prot） in control group （P＜0.05）. there were significant differences of SOD and MDA levels between high exposure group and low exposure group （P ＜0.05）. Histological examination showed that the number of spermatogenic cells and layer were decreased significantly in 2 exposure groups as compared with control group. TUNEL assay showed that apoptosis cells appeared in 2 exposure groups. Conclusion BDE-209 changed lipid peroxidation in male BALB / c mice testis and caused toxic effects on the testis.