顺式阿曲库铵在兔膈肌和外周肌的不同肌松效应

The different responses of diaphragm and peripheral muscles to cisatracurium in rabbits

目的 探索顺式阿曲库胺在兔膈肌和外周肌的不同肌松效应.方法 雄性新西兰兔8只,在戊巴比妥钠静脉麻醉下游离膈肌、前胫骨肌、比目鱼肌和膈神经、胫神经、腓总神经,并分别连接肌张力换能器和神经刺激电极.给予连续单次超强刺激,同步监测记录单次电刺激所诱发的肌颤搐张力.肌颤搐张力稳定后,采用累积剂量给药法静注顺式阿曲库铵,并通过监测的顺式阿曲库铵在膈肌、前胫骨肌和比目鱼肌的肌松效应强度,建立剂量-效应曲线关系,获得各肌群的ED50值和ED95值.结果 顺式阿曲库铵在兔膈肌、前胫骨肌和比目鱼肌作用表现为剂量依赖性抑制增强.各肌群的ED50值和ED95值分别为：膈肌（39.3±2.5）μg/kg和（75.7±4.2）μg/kg,前胫骨肌（80.6±7.5）μg/kg和（123.3±9.3）μg/kg,比目鱼肌（80.0±7.1）μg/kg和（126.9±9.4）μg/kg.膈肌与前胫骨肌和比目鱼肌比较,差异有统计学意义（P＜0.05）,前胫骨肌与比目鱼肌比较,差异无统计学意义（P＞0.05）.结论 顺式阿曲库铵在兔的膈肌和外周肌表现不同肌松作用,而在膈肌的肌松作用明显强于外周肌.

Objective To investigate the different responses of diaphragm and peripheral muscles to cisatracurium in rabbits.Methods 8 male New zealand rabbits were anaesthetized with pentobarbital,and then the diaphragm,tibialis anterior,soleus muscles,phrenic nerves,tibial nerve,and peroneal nerve were gently freeded.The muscles were secured to force displacement transducers,and the nerves were directly stimulated by electrodes with supramaximal square waves.The isometric force of twitch tention of each muscle was recorded.The cumulative dose-response technique was separately used for obtaining the ED50and ED95 values of the cisatracurium in each muscle.Results The muscle-relaxing of cisatracurium on the three muscles in were observed in a dose-dependent manner.The ED50 values and ED95 values were： diaphragm（39.3 ± 2.5）μg/kg and（75.7 ± 4.2）μg/kg,tibialis anterior（80.6 ± 7.5）μg/kg and（123.3 ±9.3）μg/kg,soleus（80.0 ± 7.1）μg/kg and（126.9 ± 9.4）μg/kg,respectively.It had significant difference between diaphragm vs tibialis or soleus,P 〈 0.05.Conclusions The muscle relaxant effects of cisatracurium on diaphragm and peripheral muscles were different,and diaphragm was more sensitive than peripheral muscles.