摘要
目的 观察关节腔注射透明质酸钠（HA）对力竭性跑台运动大鼠滑膜组织中软骨寡聚基质蛋白（COMP）表达的影响,探究滑膜中COMP对HA治疗效果是否具有评价作用.方法 将36只雄性Wistar大鼠随机分为安静对照组、力竭性运动组和力竭性运动加HA注射组,12只/组.力竭性运动加HA注射组1次/周,共5次的膝关节腔HA注射,在6周力竭性跑台运动后,取各组大鼠膝关节滑膜组织,HE染色观察其组织变化,并对其滑膜炎程度行组织学评分,应用免疫组化法检测COMP在各组大鼠滑膜组织中的表达.结果 力竭性运动加HA注射组大鼠滑膜组织滑膜炎症程度显著低于力竭性运动组大鼠（t=7.15,P＜0.01）,滑膜组织COMP的表达也显著低于安静对照组大鼠（t=6.30,P＜0.01）.结论 关节腔注射HA对膝关节滑膜炎具有治疗作用,通过检测滑膜组织中COMP的表达可以评价HA对于滑膜炎的治疗效果.
Objective To investigate the effects of intra-articular hyaluronan（HA）injection on the expression of cartilage oligomeric matrix protein（COMP）in synovium of strenuous running rats,and investigate the possibility of predicting the effectiveness of HA based on COMP in synovium.Methods 36 healthy male Wistar rats were randomly divided into control group,strenuous running group and strenuous running group and HA injection group.Strenuous running group and HA injection group were intra- articularly injected with HA once a week for 5 consecutive weeks.The histological changes of synovium of knee joint was examined by H.E.staining and immunohistochemical expression of COMP in three groups after 6 weeks＇ strenuous running.Results Synovial inflammation was less severe in strenuous running and HA injection group than strenuous running group（t =7.15,P 〈0.01）.The immunohistochemical expression of COMP in rats＇synovium of knee joint in strenuous running and HA injection group was significantly lower than that in rats＇synovium in strenuous running group（t = 6.30,P 〈 0.01）.Conclusions Intra- articular HA injection suppressed synovitis,and the expression of COMP in synovium could be used to predict the effectiveness of HA.
出处
《中国医师杂志》
CAS
2011年第3期331-333,336,共4页
Journal of Chinese Physician
基金
基金项目：辽宁省科学技术计划项目（2008225009-14）
