四川凉山地区汉族人群胰岛素受体基因第8外显子NsiⅠ多态性与2型糖尿病无关联

Insulin receptor exon 8 NsiⅠ polymorphism is not associated with type 2 diabetes mellitus in the Hans living in Liangshan,Sichuan province

目的探讨胰岛素受体(insulin receptor,INSR)基因第8外显子NsiⅠ多态性与2型糖尿病的关系。方法采用填写调查问卷,结合身高、体质量、空腹血糖、血压、三酰甘油、胆固醇、高密度脂蛋白等生理指标测定的方式,对四川凉山6个县市20岁以上汉族人群进行横断面研究。在横断面研究的基础上,以全部162例糖尿病患者为病例,按照年龄、性别与病例组进行1∶1频数匹配,从非糖尿病研究对象中随机选取162例做为对照。采用多聚酶链式反应(PCR)结合限制性内切酶方法检测基因多态性。结果 2型糖尿病组与对照组INSR基因型频率及等位基因频率差异均无显著性。结论 INSR基因第8外显子NsiⅠ多态性似与四川凉山地区汉族人群2型糖尿病无关联。

Objective To investigate the potential relationship between insulin receptor gene exon 8 Nsi Ⅰ polymorphism and type 2 diabetes mellitus.Methods A cross-sectional survey was conducted among Han residents from 6 counties,Sichuan province.Questionnaire survey,anthropometric measurement and blood test were carried out.Type 2 diabetes cases were selected case group（n=162） and healthy control individuals as control group（n=162） with frequency matching（1∶1） of sex and age.Polymerase chain reaction and restriction fragment length polymorphism（PCR-RFLP）were adopted to examine the individual genotype.Results There was no significant differences in the genotype and gene frequency between case group and controls.Conclusion Nsi Ⅰ polymorphisms is not related to type 2 diabetes in Han people of Liangshan,Sichuan province.