子宫内膜异位症不孕患者腹腔液IL-2活性及外周血T细胞亚群变化

The changes of interleukin-2 activity in peritoneal fluid and T lymphocyte subpopulation in peripheral blood of patients with endometriosis-induced infertility

目的：探讨子宫内膜异位症导致不孕的免疫机制。方法：选择临床轻度子宫内膜异位症不孕患者，以多囊卵巢综合征及不明原因不孕的患者为对照。采取CTLL细胞林法检测腹腔液IL－2生物活性，以流式细胞分选技术检测外周血T淋巴细胞亚群的变化。结果：与对照组比较，轻度子宫内膜异位症不孕患者腹腔液IL－2活性显著增高，外周血CD／T细胞数目增加，而CD8+T细胞变化不明显。结论：轻度子宫内膜异位症不孕患者腹腔免疫细胞处于高度活化状态、全身存在免疫异常。提示高水平IL-2与子宫内膜异位症导致不孕有关。

Objective: To explore the immune mechanism of endometriosis-induced infertility. Methods:The infertile women with rnild endometriosis were chosen and those with polycystic ovarian syndrome and unidentified causes as controls. The activities of IL-2 in peritoneal fluid were determined with CTLL cell line method, and the changes of T lymphocyte subpopulation in peripheral blood was also determined with flow cytornetry technique- Results: lL-2 activity was obviously increased in the infertile women with mild endometriosis compared with the controls. CDt T cell number in peripheraI blood was increased whiIe CDt T cell number had no evident changes. ConcIusion: The peritoneal immune cells were in the state of hyper activated activation and there was dysimmunity in the infertile women with mild endometriosis. lt suggest that IL-2 may play a role in endometrlosis-induced infertility.