载脂蛋白E基因多态现象与原发性骨质疏松症相关性的初步研究

A Preliminary Study on the Relationship Between Apolipoprotein E Gene Polymorphism and Primary Osteoporosis

目的 研究北京地区绝经后妇女原发性骨质疏松症与载脂蛋白Ｅ（ａｐｏｌｉｐｏｐｒｏｔｅｉｎ Ｅ，ＡｐｏＥ）基因多态现象的相关性。方法 研究对象包括３４ 例患者和３０ 例正常人，采用聚合酶链反应限制性片段长度多态性（ＰＣＲ－ ＲＦＬＰ） 分析技术检测ＡｐｏＥ 基因型。结果 ３４ 例原发性骨质疏松症患者ＡｐｏＥ２、Ｅ３ 和Ｅ４ 等位基因频率分别为０．０７４、０．６７６ 和０．２５０，而３０ 例正常对照者依次为０．１００、０．８１７和０．０８３。原发性骨质疏松症患者ＡｐｏＥ４ 等位基因频率明显高于正常对照者（χ２ ＝ ６．２２，Ｐ＜ ０．０５）。结论ＡｐｏＥ４ 等位基因与绝经后妇女原发性骨质疏松症密切相关。ＡｐｏＥ基因型是原发性骨质疏松症一个有用的标志物。

Objective To Study the relationship between apolipoprotein E gene polymorphism and primary osteoporosis in postmenopausal Chinese women in Beijing. Methods 34 patients and 30 normal controls were included. ApoE genotypes were determined with the method of polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP). Results The frequencies of ApoE2,E3 and E4 alleles were respectively 0.074, 0.676 and 0.250 in 34 patients, and 0.100, 0.817 and 0.083 in 30 controls. The frequency of E4 was significantly higher in primary osteoporotic patients than that in the normal controls (χ2=6.22, P< 0.05). Conclusion The ApoE4 allele is associated with primary osteoporosis in postmenopausal Chinese women, ApoE genotype might be a useful marker for primary osteoporosis in Chinese postmenopausal female.