期刊文献+

泛耐药鲍曼不动杆菌相关耐药基因检测及分析 被引量:7

Detection and analysis of antibiotic-resistance-related genes in pan-drug resistant A.baumannii
下载PDF
导出
摘要 【目的】了解泛耐药鲍曼不动杆菌(PDR-AB)10簇β-内酰胺酶(bla)基因、2簇碳青霉烯酶基因(OXA)、2簇氨基糖苷类修饰酶(AMEs)基因、3种整合子酶基因和消毒剂-磺胺类耐药基因携带情况。【方法】用聚合酶链反应(PCR)及序列分析方法对30株从我院分离的PDR-AB进行各种耐药基因的检测和分析。【结果】在30株PDR-AB中blaADC阳性30株(100%),blaSHV阳性29株(97%),blaVIM阳性26株(87%),blaIMP阳性24株(80%),blaPER阳性14株(47%),blaTEM阳性14株(47%),blaDHA阳性12株(40%),blaGES阳性8株(27%)b,laGIM阳性6株(20%);OXA-23阳性27株(90%);Ant(3’’)-I阳性30株(100%)I;ntI1阳性30株(100%)I,ntI2阳性8株(27%)I,ntI3阳性2株(7%);QacE△1-sull阳性30株(100%);没有检测到blaVEB、OXA-24和Aac(6’)-II基因。【结论】泛耐药鲍曼不动杆菌同时携带多种耐药基因是导致其对所有常用药物均耐药的重要原因,临床应加强有效的监测和隔离工作来控制其传播。 [Objective] To investigate the existence of the genes encoding ten beta-lactamases(bla), two carbapenemases(OXA), two aminoglycosides-modifying enzymes(AMEs), genes of three integrases and disinfectants-sulfanilamide-resistance gene in pan-drug resistant Acinetobacter baumannii(PDR-AB). [ Methods] Genetypes of thirty strains of PDR-AB were analyzed by polymerase chain reaction(PCR)and verified by DNA sequencing. [ Results ] The positive rates of blaADC, blaSHV,blaVIM ,blaIMP,blaPER,blaTEM, blaDHA , blaGES, blaGIM, OXA-23, Ant(3 ' ' )-I, IntI 1, IntI2, IntI3 and QacE △ 1 -sull were respectively 100%, 97% 、 87%、 80% 、47%、 47% 、40%、 27%、20%、 90%、100%、100%、 27%、 7% and 100%, blaVEB, OXA-24 and Aac(6 ')-II weren' t detected. [ Gonclusion ] The pan-drag resistant Acinetobacter baumannii was cm'rying so many antiblotic-reslstance-related genes, which was the most important causing that led to resistance to all drugs used commonly. So effective monitoring and quarantine work should be strengthened to control the epidemic of these strains.
机构地区 解放军
出处 《武警医学院学报》 CAS 2011年第5期343-346,共4页 Acta Academiae Medicinae CPAPF
关键词 泛耐药鲍曼不动杆菌 耐药基因 聚合酶链反应 Pan-drug resistant acinetobacter baumannii Resistant genes Polymerase chain reaction
  • 相关文献

参考文献21

二级参考文献130

共引文献451

同被引文献113

  • 1胡芳,付英梅,马佳毓.整合子与细菌耐药性[J].微生物学杂志,2006,26(2):79-81. 被引量:4
  • 2糜祖煌.细菌抗菌药物耐药基因研究中值得注意的问题(Ⅱ)[J].世界感染杂志,2007,7(4):267-268. 被引量:3
  • 3Piaa O, Iasiello A, Papalanni C, et al. Incidence of antimicrobial- resistant ventilator associated pneumonia: an eighteen-month survey. Panminerva Med, 2005, 47(4): 265-267.
  • 4Tekqe AY, Erbay A, Cabadak H, et al. Pan-resistant Ac/netobacter baumann/i mediastinitis treated successfully with tigecycline: a case report. Surg Infect (Larehmt), 2011: 12(2): 141-143.
  • 5Khalaf NG, Eletreby MM, Hanson ND. Characterization of CTX-M ESBLs in Enterobacter cloacae, Escherichia coli and Klebsiella pneu- mon/ae clinical isolates from Cairo, Egypt. BMC Infect Dis, 2009, 9:84.
  • 6Park YK, Peck KR, Cheong HS, et al. Extreme drug resistance in Acinetobacter baumannii infections in intensive care units, South Ko- rea. Emerg Infect Dis, 2009, 15(8): 1325-1327.
  • 7Telang NV, Satpute MG, Dhakephalkar PK, et al. Fulminating sep- ticemia due to persistent pan-resistant community-acquired metaUo-- lactamase (IMP-1)-positive Acinetobacter baumann/i. Indian J Pathol Microbiol, 2011, 54(1): 180-182.
  • 8Stokes HW, Hall RM. A novel family of potentially mobile DNA elements encoding site-specific gene-integration func- tions: integrons[J]. Mol Microbio, 1989, 3(12): 1669-1683.
  • 9Hall RM, Brookes DE, Stokes HW. Site-specific insertion of genes into integrons: role of the 59-base element and deter- mination of the recombination cross-over point[J]. Mol Mi- crobiol, 1991, 5(8):1941-1959.
  • 10Goldstein C, Lee M D, Sanchez S, et al. Incidence of class 1 and 2 integrases in clinical and commensal bacteria from livestock, companion animals, and exotics[J]. Antimicrob Agents Chemother, 2001, 45(3):723-726.

引证文献7

二级引证文献19

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部