基于药物成分体内过程的黄连苦寒药性研究

Study the cold-bitter property of Coptis chinensis Franch. based on processes of metabolism disposition in intra-body

目的:利用现代研究手段诠释黄连苦寒药性的本质。方法:以指纹图谱为表征手段,利用HPLC-UV技术,通过体外药材图谱、体内血清图谱及尿液图谱间的比较,综合评价黄连体内直接作用的物质基础,探讨其给药后成分与传统疗效的相关性,并通过比较黄连在热病状态下与生理状态下的药物成分吸收情况,从而阐明代表其苦寒药性的成分群。结果:黄连入血的全部9个可检测出的成分及在黄连药材和尿液中可检测出的3个成分(体外成分图谱中4、5、6号峰),可以认定为黄连的药效物质基础,亦可能代表其苦寒药性。结论:基于药物成分体内过程的研究方法在一定程度上可阐释黄连苦寒药性。

Objective：To study the essence of the cold-bitter property of Coptis chinensis Franch.（CCF） through applying to the modern means.Methods：Using HPLC-UV techniques,the fingerprints in vitro,plasma and urine samples after oral administration of CCF were compared,to comprehensively evaluate effective substances basis in vivo of CCF,explore the relevance of the compounds after oral administration and traditional effects.Through comparing the specific absorbance of the components in CCF under pathology and physiology status,the ingredient groups of its cold-bitter property were clarified.Results：All 9 compounds were detected which absorbed into blood from CCF and 3 compounds（peak 4,5,6 in the chromatogram of CCF in vitro） detected in vitro and urine.These compounds may be concluded as effective substances basis and also be represented cold-bitter property of CCF.Conclusion：The cold-bitter property of CCF can be explained by processes of metabolism and disposition on its compounds in intra-body.