大黄虫丸超微粉剂对肾间质纤维化模型大鼠血流变的影响

Effects of ultrafine powder of Daihuang Zhechong pill on hemorheology in rats with renal interstitial fibrosis

目的:探讨大黄虫丸超微粉剂对肾间质纤维化模型大鼠血流变的影响,并论证其抗纤维化机制。方法:通过单侧输尿管结扎术建立肾间质纤维化大鼠模型,观察大黄虫丸超微粉剂对该模型大鼠血清尿素氮(BUN)、肌酐(SCr)、透明质酸酶(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(CⅣ)、全血黏度150/s切变率、30/s切变率、5/s切变率、1/s切变率以及血细胞比容的影响。结果:大黄虫丸超微粉剂能显著降低肾间质纤维化大鼠血清BUN、SCr、HA、LN、PCⅢ、CⅣ以及全血黏度150/s切变率、30/s切变率、5/s切变率、1/s切变率、血细胞比容指数。结论:大黄虫丸超微粉剂对肾间质纤维化大鼠血流变的改善,进一步证明了活血化瘀是其抗肾间质纤维化机制之一。

Objective：To explore the effects of ultrafine powder of Daihuang Zhechong pill（UPDHZCP） on hemorheology in rats with renal interstitial fibrosis（RIF）,and further demonstrate the mechanism of UPDHZCP in antagonizing RIF.Methods：The RIF models were established by the unilateral ureteral obstruction（UUO）,and to observe the influence of UPDHZCP on the serum urea nitrogen（BUN）,creatinine（SCr）,hyaluronidase（HA）,laminin（LN）,Ⅲ pro-collagen（PCⅢ）,Ⅳ collagen（CⅣ）,blood viscosity at shear rate in 150/s,30/s,5/s,1/s and the hematocrit.Results：UPDHZCP distinctly could reduce the indexes of serum BUN,SCr,HA,LN,PCⅢ,CⅣ,blood viscosity which at a different shear rate and hematocrit in RIF rats.Conclusion：UPDHZCP can improve the hemorheology in rats with RIF,promoting blood circulation and removing blood stasis method is one of the mechanism for it in antagonizing RIF.