低分子肝素对酒精性肝损伤大鼠的影响

Effects of low molecular weight heparin on the rat model of alcoholic liver disease

目的研究低分子肝素对酒精性肝损伤大鼠的影响。方法制备大鼠酒精性肝损伤模型,成模后部分大鼠小剂量低分子肝素(100 IU/kg)皮下注射进行干预,4周后分别取血和肝组织,从病理组织学、超微结构及分子免疫学等方面观察低分子肝素对酒精性肝损害大鼠肝脏的影响。结果与模型组比较,低分子肝素治疗4周后,大鼠血清天门冬氨酸氨基转移酶、总胆固醇、低密度脂蛋白含量明显下降(P<0.01);酶联免疫吸附法(ELISA)结果显示,血清瘦素水平显著降低(P<0.05);光学、电子显微镜观察见脂肪变性、炎症反应、纤维化程度明显减轻(P<0.01)。结论低分子肝素通过改善脂肪代谢、降低瘦素表达而对大鼠酒精性肝损伤起到一定的保护治疗作用。

Objective To study the effect of low molecular weight heparin on alcohol-fed rats. Methods The rat model of alcoholic liver disease was established by alcohol lavage,and then treated with low molecular weight heparin（100 IU/kg）.After 4 weeks,blood and liver tissue samples were collected.The effect of low molecular weight heparin on alcoholic liver disease rats was observed by pathology,ultrastructures and molecular immunology.Results Compared with the model group,the low molecular weight heparin therapy group significantly improved the levels of aspartate aminotransferase,total cholesterol and low density lipoprotein（P0.01）,while it lowered the serum leptin level（P0.05）.Under observation by optical and electronic microscopes,degrees of liver steatosis,inflammation reaction and fibrosis were significantly reduced（P0.01）.Conclusion Low molecular weight heparin has a therapeutic effect on alcohol-induced liver disease rats by improving fat metabolism and reducing serum leptin.