灯盏乙素对MMT诱导SK-N-SH神经细胞凋亡的保护作用

Inhibitory effect of scutellarin on apoptosis induced by MMT in SK-N-SH neural cells

目的研究灯盏乙素对新型大气污染物甲基环戊二烯羰基锰(MMT)诱导人SK-N-SH细胞凋亡的保护作用及可能机制。方法将SK-N-SH细胞随机分为5组:正常组,MMT诱导组,1.2×10-1mg/L灯盏乙素组,3.0×10-2mg/L灯盏乙素组,7.5×10-3mg/L灯盏乙素组。灯盏乙素预保护2 h,1 mmol/L MMT诱导24 h后,MTT法检测细胞存活率,分光光度法检测细胞活性氧(ROS)生成、细胞培养液和细胞内超氧化物歧化酶(SOD)活性及丙二醛(MDA)产量;化学发光法检测细胞三磷酸腺苷(ATP)释放量及Caspase-3活性变化。结果灯盏乙素预保护的细胞在MMT诱导后,与MMT诱导组相比,细胞存活率上升(P<0.05),细胞ROS生成量受到抑制(P<0.01);细胞培养液和细胞内SOD活性增高(P<0.05),MDA产量降低(P<0.01);在这一过程中,细胞ATP的释放量有所恢复(P<0.05),同时Capase-3活性受到抑制(P<0.05)。结论灯盏乙素通过抗氧化作用,抑制MMT对SK-N-SH细胞的凋亡作用。

Objective To explore the inhibitory effect of scutelalrin on methylcyclopentadienyl manganese tricarbonyl（MMT）-induced apoptosis in SK-N-SH cells and its possible mechanism.Methods SK-N-SH cells were randomly grouped into 5 groups： the normal group,the MMT induction group,the 1.2×10-1mg/L scutellarin group,the 3.0×10-2mg/L scutellarin group and the 7.5×10-3mg/L scutellarin group.After incubation with scutelalrin for 2 h,SK-N-SH cells were induced by 1 mmol/L of MMT for 24 h.Then viability of cells was determined the MTT assay.The production of reactive oxygen species（ROS） and malondialdehyde（MDA） and activity of superoxide dismutase（SOD） were determined by a spectrophotometer.The release of adenosine triphosphate（ATP） and activity of Caspase-3 were measured by chemiluminescence.Results After incubation with scutelalrin,the viability of cells markedly ascended（P0.05）.The production of ROS and MDA was inhibited（P0.01）,while the activity of SOD was increased（P0.05）.The release of ATP partly resumed（P0.05）,while the activity of Caspase-3 was significantly inhibited（P0.01）.Conclusion Scutelalrin can inhibit apoptosis of SK-N-SH cells induced by MMT through anti-oxidation in this process.