HepaCAM在肾透明细胞癌中的表达及临床意义

Expression and clinical significance of HepaCAM expression in renal clear cell carcinoma

目的检测肾透明细胞癌(RCCC)组织中肝细胞黏附因子(HepaCAM)的表达水平,探讨HepaCAM在肾透明细胞癌发生、发展过程中的意义。方法选取73例肾透明细胞癌组织和12例癌旁组织作为研究对象,采用非生物素即用型二步法(PV-9001)免疫组织化学方法检测组织中HepaCAM蛋白的表达,运用Image-Pro Plus 6.0图像分析软件测定免疫组化图像的累积光密度值(IOD),应用SPSS13.0软件进行统计分析。结果 HepaCAM蛋白在RCCC组织中低表达,而在癌旁组织中高表达,IOD分别为8203.87±8431.88和36639.28±20851.94,差异具有统计学意义(P<0.05)。而HepaCAM蛋白的表达与性别、年龄、肿瘤大小、T分期和TNM分期等临床特征均无相关关系。结论 HepaCAM蛋白在癌组织中低表达,可能与肾透明细胞癌的发生有密切关系,可作为判断肿瘤发生的指标。

Objective To detect expression of hepatocyte cell adhesion molecule（HepaCAM） in renal clear cell carcinoma（RCCC）,and to study the relativity of HepaCAM expression with occurrence and development of RCCC.Methods Paraffin-embedded tissue sections from 73 patients with RCCC and 12 RCCC adjacent renal tissues were included in the study.Expression of HepaCAM in all the samples was analyzed with the immunohistochemical（PV-9001） method.Integrated optical density（IOD） of the immunohistochemical image was estimated by Image-ProPlus 6.0 software,and the correlation of IOD and clinical features of RCCC was analyzed.Statistical analysis was performed by SPSS13.0 software.Results HepaCAM expression was down-regulated in RCCC,while it was up-regulated in RCCC adjacent tissues.IOD in the two groups was 8 203.87±8 431.88 and 36 639.28±2 0851.94,respectively（P0.05）.Expression of HepaCAM had no relation with clinical features such as gender,age,the tumor size,the T stage and TNM stage.Conclusions HepaCAM expression is lower in RCCC compared with RCCC adjacent tissues,and may has a close relation with the development of RCCC.HepaCAM is supposed to act as a marker for carcinogenesis in the kidney.