NMDA受体亚单位(NR2A、NR2B)在正常视觉发育与屈光参差性弱视猫模型初级视皮质表达的研究

Roles and Expressions of the NMDA Receptor Subunits (NR2A and NR2B) in Visual Cortex Area of Kittens with the Normal Visual Development and Anisometropic Amblyopia

本实验主要采用免疫组织化学SP法,研究不同视觉发育阶段屈光参差性弱视猫和正常猫大脑初级视皮质17区N-甲基-D-天门冬氨酸(NMDA)受体亚单位NR2A、NR2B的表达变化,探讨NR2A、NR2B与视觉发育可塑性的联系及屈光参差对两种受体亚单位表达产生的影响。结果发现NR2A、NR2B随发育变化,视觉发育关键期高峰期后下降,并维持一定水平;屈光参差弱视猫模型视皮质17区NR2A下调,NR2B视觉发育成熟期前变化不明显,后有上升趋势。可见屈光参差可导致初级视皮质NR2A表达下调,而NR2B表达在视觉发育成熟期后有上调趋势;提示NR2A和NR2B表达比例构成的差异可能是参与屈光参差性弱视发病的分子机制之一。

In order to understand the roles of the other subunits,we investigated expression of the NMDA receptor subunits（NR2A and NR2B） in visual cortex of normal and anisometropic amblyopia kittens with different ages in the present study.We examined the expressions of NR2A and NR2B in the visual cortex of the kittens by immunohisto-chemstry with polyclonal anti-NR2A antibody and anti-NR2B antibody,respectively.Using immunohisto-chemical Streptavidin Perosidase（SP） method,we observed the dynamic changes of NR2A and NR2B with microscope and computer-assisted image analyses.We found that NR2A and NR2B remained low expression after the peak of the critical period of kitten visual development;compared with normal group of the same age,NR2A expresses low.However,the difference is not significant for NR2B before maturation period of visual development.NR2B rises after the maturation period of visual development.According to this,the component of NR2A and NR2B can be affected by anisometropia.This research suggests that the difference of NR2A and NR2B expressions may affect the formation of amblyopia.