脂联素水平及基因多态性与冠心病的关系

Relationships between Adiponectin level and its Gene Polymorphism with Coronary Heart Disease

目的:探讨脂联素(APN)水平及其单核苷酸基因多态性(SNP)+45T/G和+276G/T 2个位点与冠心病(CHD)的关系。方法:将195例研究对象分为正常对照组和CHD组,采用实验-对照研究方法,应用酶联免疫吸附法(ELISA)及聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术,对血浆APN浓度及APN 2个位点基因多态性进行检测。结果:2组比较,CHD组APN降低(P<0.05);CHD组SNP+45TG+GG基因型和G等位基因的分布频率明显低于正常对照组,差异具有统计学意义(P<0.05);基因SNP+276多态性位点各基因型频率与等位基因频率在2组之间差异均无统计学意义(P>0.05)。结论:CHD患者APN水平下降,SNP+45G等位基因可能为CHD的保护因素;SNP+276位点基因多态性可能与CHD的发生无明显相关性。

Objective：To pursue the relationship between Adiponectin blood level and its single nucleotide polymorphism（SNP） ＋45 T/G and ＋276 G/T with Coronary Heart Disease（CHD）.Method：Firstly,195 patients were divided into normal control（CO） group and coronary heart disease（CHD） group,and then according to experimental-control study method,Enzyme Linked Immunosorbent Assay（ELISA） and Polymerase Chain Reaction Restriction Fragment Length Polymorphism（PCR-RELP） were used to detect the level of plasma adiponection and polymorphisms of its two polymorphism sites ＋45 T/G and ＋276 G/T.Result：In the two groups,APN of CHD group was reduced（P0.05）,and Distribution Frequency of SNP ＋45 TG ＋GG genotype and G allele of CHD group was also significantly lower than control group,further,the difference was statistically significant（P0.05）;On the other hand,the difference of gene SNP ＋276 polymorphism and allele frequence of the genotype frequencies between two groups were showed no significant difference（P0.05）.Conclusion：APN level of patients was lower in CHD group,and SNP ＋45 G allele might be the protective factor for CHD,and more,SNP ＋276 polymorphism might not be associated with occurrence of CHD.