摘要
AIM:To investigate the differences in cultivable gut bacteria and peroxisome proliferator-activated receptor γ2(PPAR-γ2) gene Pro12Ala variation in obese and normal-weight Chinese people.METHODS:Using culture methods,the amounts of Escherichia coli,Enterococci,Bacteroides,Lactobacilli,Bif idobacteria and Clostridium perfringens(C.perfringens) in the feces of 52 obese participants [body mass index(BMI):≥ 28 kg/m2] and 52 participants of normalweight(BMI:18.5-24 kg/m2) were obtained.Study participants completed comprehensive questionnaires and underwent clinical laboratory tests.The polymerase chain reaction-restriction fragment length polymorphism(PCR-PFLP) assay was used to analyze PPAR-γ2 gene Pro12Ala variation.RESULTS:The obese group exhibited a lower amount of C.perfringens(6.54 ± 0.65 vs 6.94 ± 0.57,P = 0.001)and Bacteroides(9.81 ± 0.58 vs 10.06 ± 0.39,P = 0.012) than their normal-weight counterparts.No major differences were observed in Pro12Ala genotype distribution between the two groups;however,obese individuals with a Pro/Ala genotype had a signif icantly lower level of Bacteroides(9.45 ± 0.62 vs 9.93 ± 0.51,P = 0.027) than those with a Pro/Pro genotype.In addition,the obese group demonstrated a higher stool frequency(U = 975,P < 0.001) and a looser stool(U = 1062,P = 0.015) than the normal-weight group.CONCLUSION:Our results indicated interactions among cultivable gut flora,host genetic factors and obese phenotype and this might be helpful for obesity prevention.
AIM：To investigate the differences in cultivable gut bacteria and peroxisome proliferator-activated receptor γ2（PPAR-γ2） gene Pro12Ala variation in obese and normal-weight Chinese people.METHODS：Using culture methods,the amounts of Escherichia coli,Enterococci,Bacteroides,Lactobacilli,Bif idobacteria and Clostridium perfringens（C.perfringens） in the feces of 52 obese participants [body mass index（BMI）：≥ 28 kg/m2] and 52 participants of normalweight（BMI：18.5-24 kg/m2） were obtained.Study participants completed comprehensive questionnaires and underwent clinical laboratory tests.The polymerase chain reaction-restriction fragment length polymorphism（PCR-PFLP） assay was used to analyze PPAR-γ2 gene Pro12Ala variation.RESULTS：The obese group exhibited a lower amount of C.perfringens（6.54 ± 0.65 vs 6.94 ± 0.57,P = 0.001）and Bacteroides（9.81 ± 0.58 vs 10.06 ± 0.39,P = 0.012） than their normal-weight counterparts.No major differences were observed in Pro12Ala genotype distribution between the two groups;however,obese individuals with a Pro/Ala genotype had a signif icantly lower level of Bacteroides（9.45 ± 0.62 vs 9.93 ± 0.51,P = 0.027） than those with a Pro/Pro genotype.In addition,the obese group demonstrated a higher stool frequency（U = 975,P 〈 0.001） and a looser stool（U = 1062,P = 0.015） than the normal-weight group.CONCLUSION：Our results indicated interactions among cultivable gut flora,host genetic factors and obese phenotype and this might be helpful for obesity prevention.
基金
Supported by Danone Institute China Diet Nutrition Research and Communication grant (2006)
