松生拟层孔菌提取物对肝癌细胞H22和肉瘤细胞S180的抑制作用

Inhibitory effect of Fomitopsis pinicola extract on hepatocellular carcinoma H22 cells and sarcoma S180 cells

目的:观察松生拟层孔菌(Fomitopsis pinicola,FP)乙醇提取物Ⅰ号(简称FP-Ⅰ)的体内外抗肿瘤活性及其可能的机制。方法:以乙醇提取FP,获取乙酸乙酯相即为FP-Ⅰ。MTT法检测FP-I体外对小鼠肝癌细胞株H22及小鼠肉瘤细胞株S180增殖的抑制作用;建立S180细胞小鼠移植瘤模型,检测各组小鼠瘤质量、抑瘤率、胸腺指数、脾脏指数、外周血白细胞数及淋巴细胞比例、肿瘤红细胞花环形成率,H-E染色观察FP-Ⅰ对各组S180移植瘤细胞凋亡的影响及心、肝、肾、胸腺和脾脏的组织学变化。结果:50、100、200、400μg/ml FP-Ⅰ对S180细胞增殖的抑制率分别为22.35%、32.49%、40.01%和74.01%,对H22细胞的抑制率分别为45.19%、51.10%、66.37%和82.40%。25、50、100 mg/kg FP-Ⅰ对小鼠S180移植瘤生长的抑瘤率分别为79.92%、66.18%和78.45%,CTX阳性对照(30 mg/kg)的抑瘤率为84.10%;中、高剂量FP-Ⅰ组S180荷瘤小鼠的淋巴细胞比例、胸腺指数及红细胞花环率均有一定程度的提高(P<0.05或P<0.01);各剂量组的S180移植瘤细胞均出现细胞凋亡的典型形态变化。结论:松生拟层孔菌提取物具有较强的抗肿瘤活性,其机制与其增强小鼠免疫功能和诱导肿瘤细胞凋亡有关。

Objective：To study the in vitro and in vivo anti-tumor activities of Fomitopsis pinicola extract and their possible mechanisms.Methods： Fomitopsis pinicola was extracted by alcohol and the resulting acid ethyl esters extract was named FP-I.The inhibitory effects of FP-I on proliferation of mouse hepatocellular carcinoma H22 cells and mouse sarcoma S180 cells were detected by MTT assay in vitro.S180-implanted mouse model was established,and the tumor weight,tumor inhibitory rate,thymus index,spleen index,peripheral leukocyte number,peripheral lymphocyte rate,and associate tumor erythocyte rosette rate（ATER） were examined in different group.Effect of FP-I on apoptosis of S180-implanted tumor cells and the histological changes of the heart,liver,kidney,thymus and spleen were observed by H-E staining.Results： The inhibitory rates of FP-I（50,100,200,400 μg/ml） on proliferation of S180 cells were 22.35%,32.49%,40.01%,and 74.01%,respectively;and those on H22 cells were 45.19%,51.10%,66.37%,and 82.40%,rspectively.The inhibitory rates of FP-I（25,50,100 μg/ml） on growth of S180-implanted tumors were 79.92%,66.18% and 78.45%,respectively,and that of CTX（30 mg/kg） positive control was 84.10%.Proportion of lymphocyte in periphoral blood,thymus index and ATER of S180-bearing mice were significantly increased in both the high-and medium-dose FP-I groups（P〈0.05 or P〈0.01）.S180-implanted tumor cells in all FP-I treatment groups showed typical apoptotic morphology.Conclusion： Fomitopsis pinicola extract has anti-tumor activity,which might relate to its ability to improve the immune function of mice and to induce apoptosis of tumor cells.