Survivin参与胰腺癌PaTu8988细胞对吉西他滨的耐药

Survivin is involved in drug resistance of pancreatic cancer PaTu8988 cells to gemcitabine

目的:探讨凋亡抑制蛋白survivin在胰腺癌细胞株PaTu8988中的表达,及其与吉西他滨(gemcitabine,GEM)耐药的关系。方法:MTT法检测GEM(0.01、0.1、1.0、2.5、5.0、10.0μg/ml)对PaTu8988细胞生长的抑制作用,流式细胞术检测GEM作用后PaTu8988细胞的凋亡率,RT-PCR检测survivin mRNA在PaTu8988细胞中的表达。结果:高质量浓度GEM(≥1.0μg/ml)可显著抑制PaTu8988细胞的增殖、促进PaTu8988细胞的凋亡;而低质量浓度GEM(0.01、0.1μg/ml)作用不明显。低质量浓度GEM时间依赖性地上调PaTu8988细胞中survivin mRNA的表达;而质量高浓度GEM作用PaTu8988细胞后,sur-vivin mRNA的表达48 h时逐渐下降,而后逐渐上升。结论:胰腺癌PaTu8988细胞中survivin mRNA高表达,可能是其对GEM产生耐药的原因之一。

Objective：To explore the expression of apoptotic inhibitory protein survivin in pancreatic cancer cell line PaTu8988, and to study its role in the drug resistance of PaTu8988 cells to gemeitabine （GEM）. Methods： The inhibitory effect of GEM （0.01,0.1, 1.0, 2.5, 5.0, and 10.0 μg/ml） on PaTu8988 cells was detected by MTT assay; apoptosis rate of PaTu8988 cells treated with GEM was determined by flow eytometry; and the survivin mRNA expression in PaTu8988 cells was examined by RT-PCR. Results： High dosage of GEM （ ≥ 1.0μg/ml） greatly inhibited growth and promoted apoptosis of PaTu8988 cells, while low dosage of GEM （0.01, 0. 1 μg/ml） showed no effects. Low dose of GEM time-dependently increased expression of survinin mRNA in PaTu8988 cells ; high dosage of GEM gradually inhibited survivin mRNA expression within the first 48 h, and then survivin mRNA expression gradually increased as time went by. Conclusion： Survivin mRNA is highly expressed in pancreatic cancer cell line PaTu8988, which may be one of the reasons for drug resistance to GEM.