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硫化氢对THP-1源性巨噬细胞脂质摄取的影响 被引量：2

Hydrogen Sulfide Inhibits ox-LDL Uptake in THP-1-Derived Macrophages
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摘要 目的探索硫化氢对THP-1源性巨噬细胞脂质摄取的影响及可能机制。方法采用荧光显微镜测DiI标记的氧化型低密度脂蛋白(DiI-ox-LDL)摄取、RT-PCR和免疫印迹法测定CD36 mRNA和蛋白表达。结果巨噬细胞与DiI-ox-LDL孵育后大量摄取氧化型低密度脂蛋白(ox-LDL),外源性硫化氢供体硫氢化钠显著抑制巨噬细胞摄取氧化型低密度脂蛋白,而炔丙基甘氨酸(一个硫化氢产生酶胱硫醚-γ-裂解酶抑制剂)加剧巨噬细胞摄取氧化型低密度脂蛋白。进而,硫氢化钠呈浓度依赖性抑制巨噬细胞CD36 mRNA和蛋白表达,而炔丙基甘氨酸促进巨噬细胞CD36 mRNA和蛋白表达。结论硫化氢抑制巨噬细胞摄取脂质及清道夫受体CD36表达。 Aim To investigate the effects of hydrogen sulfide（H2S） on ox-LDL uptake of THP-1-derived macrophages and its underlying mechanisms. Methods Fluorescence microscopy,RT-PCR and western blot were performed. Results Incubation of macrophages with DiI-ox-LDL led to ox-LDL uptake of macrophages.Sodium hydrosulfide（NaHS,an H2S donor） decreased ox-LDL uptake. While DL-propargylglgycine（PPG） exerted opposite effects.Furthermore,ox-LDL markedly induced CD36 mRNA and protein expression in macrophages,which was abolished by NaHS（50 to 100 μmol/L）,but enhanced by PPG. Conclusions H2S inhibits ox-LDL uptake of THP-1-derived macrophages and down-regulates CD36 mRNA and protein expression.
作者 赵战芝 姜志胜 易光辉 谭建苗 邓华菲 索荣
机构地区 南华大学心血管病研究所暨湖南省动脉硬化学重点实验室 南华大学机能学实验中心 湘南学院病理生理教研室
出处 《中国动脉硬化杂志》 CAS CSCD 北大核心 2011年第2期85-88,共4页 Chinese Journal of Arteriosclerosis
基金 国家自然科学基金(30971169) 湖南省自然科学基金项目(09JJ6044) 湖南省科技计划项目(2008TP4038-2) 湖南省研究生创新项目(2008CXJJ04) 湖南省高校科技创新团队支持计划项目(2008-244)
关键词 硫化氢 THP-1源性巨噬细胞 氧化型低密度脂蛋白 脂质摄取 Hydrogen Sulfide THP-1-Derived Macrophages Oxidized Low Density Lipoprotaein Lipid Uptake
分类号 R363 [医药卫生—病理学]
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参考文献12

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二级参考文献17

  • 1闫辉,杜军保,唐朝枢.自发性高血压大鼠硫化氢/胱硫醚γ-裂解酶体系的实验观察[J].中华医学杂志,2004,84(13):1114-1117. 被引量：34
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共引文献3

同被引文献32

  • 1周小琴,成蓓,袁永辉.ATP结合盒转运子A1对巨噬细胞载脂蛋白E分泌的调节作用[J].中国病理生理杂志,2004,20(7):1149-1152. 被引量：1
  • 2Yanfei Wang,Xia Zhao,Hongfang Jin,Hongling Wei,Wei Li,Dingfang Bu,Xiuying Tang,Yali Ren,Chaoshu Tang,Junbao Du.Role of Hydrogen Sulfide in the Development of Atherosclerotic Lesions in Apolipoprotein E Knockout Mice[J]. Arteriosclerosis, Thrombosis, and Vascular Biology . 2009 (2)
  • 3Suowen Xu,Zhiping Liu,Peiqing Liu.Targeting hydrogen sulfide as a promising therapeutic strategy for atherosclerosis[J]. International Journal of Cardiology . 2014
  • 4Sisi Lin,Chun Zhou,Edward Neufeld,Yu-Hua Wang,Suo-Wen Xu,Liang Lu,Ying Wang,Zhi-Ping Liu,Dong Li,Cuixian Li,Shaorui Chen,Kang Le,Heqing Huang,Peiqing Liu,Joel Moss,Martha Vaughan,Xiaoyan Shen.BIG1, a Brefeldin A–Inhibited Guanine Nucleotide-Exchange Protein Modulates ATP-Binding Cassette Transporter A-1 Trafficking and Function[J]. Arteriosclerosis, Thrombosis, and Vascular Biology . 2013 (2)
  • 5Roshni R. Singaraja,Henk Visscher,Erick R. James,Angeliki Chroni,Jonathan M. Coutinho,Liam R. Brunham,Martin H. Kang,Vassilis I. Zannis,Giovanna Chimini,Michael R. Hayden.Specific Mutations in ABCA1 Have Discrete Effects on ABCA1 Function and Lipid Phenotypes Both In Vivo and In Vitro[J]. Circulation Research . 2006 (4)
  • 6P. Kamoun.Endogenous production of hydrogen sulfide in mammals[J]. Amino Acids . 2004 (3)
  • 7Junbao Du,Yan Hui,Yiufai Cheung,Geng Bin,Hongfeng Jiang,Xiaobo Chen,Chaoshu Tang.The possible role of hydrogen sulfide as a smooth muscle cell proliferation inhibitor in rat cultured cells[J]. Heart and Vessels . 2004 (2)
  • 8Lingyun Wu,Wei Yang,Xuming Jia,Guangdong Yang,Dessislava Duridanova,Kun Cao,Rui Wang.Pancreatic islet overproduction of H2S and suppressed insulin release in Zucker diabetic rats. Laboratory Investigation . 2008
  • 9Liping Fang,Hong Li,Chaoshu Tang,Bin Geng,Yongfen Qi,Xinmin Liu.Hydrogen sulfide attenuates the pathogenesis of pulmonary fibrosis induced by bleomycin in rats. Canadian Journal of Physiology and Pharmacology . 2009
  • 10Zhao W,Zhang J,Lu Y,Wang R.The vasorelaxant effect of H(2)S as a novel endogenous gaseous K(ATP) channel opener. EMBO Journal . 2001

引证文献2

二级引证文献5

中国动脉硬化杂志
2011年 第2期

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