Toll样受体9对巨噬细胞脂周素2表达的影响

The Effect of ODN1826,a TLR9 Ligand,on the Expression of Perilipin2 in Macrophages

目的研究ODN1826对巨噬细胞脂周素2表达的影响,并揭示其调控的相关分子机制。方法应用半定量PCR和免疫印迹法分别测定ODN1826对脂周素2 mRNA和蛋白水平表达影响。应用荧光素酶活性分析方法检测ODN1826对脂周素2启动子活性的影响。结果 ODN1826以剂量和浓度依赖方式上调脂周素2 mRNA和蛋白水平表达,并且通过Ets/AP-1位点促进脂周素2启动子活性。结论在巨噬细胞中,TLR9信号显著诱导了脂周素2 mRNA和蛋白的表达,并且脂周素2启动子区域的Ets/AP-1位点参与了这一诱导过程。

Aim To investigate the effect of ODN1826,a TLR9 ligand,on perilipin 2 expression in macrophages. Methods Real-time PCR and Western blotting were performed to detect perilipin 2 expression.Transient transfection and luciferase assay were employed to measure the perilipin 2 promoter activity. Results ODN1826 significantly induced perilipin 2 expression in a dose and time dependent manner in macrophages.ODN1826 markedly enhanced perilipin 2 promoter activity,and this process requires Ets/AP-1 activity. Conclusions TLR9 signal pathway stimulated perilipin 2 expression in macrophages,in addition,ODN1826-induced perilipin 2 promoter activity requires Ets/AP-1 activity.