钙敏感受体在动脉粥样硬化大鼠血管内皮细胞中的表达

Expression of Calcium Sensing Receptor in Atherosclerosis Rat Vascular Endothelial Cells

目的探讨钙敏感受体在动脉粥样硬化大鼠胸主动脉血管内皮细胞中的表达及其与细胞凋亡的关系。方法 Wistar大鼠144只,随机分为对照组和动脉粥样硬化组,每组72只。动脉粥样硬化早期模型复制首先每千克体重注射维生素D360万单位,然后喂养高脂饲料6周。自动生化仪检测血清甘油三酯、总胆固醇;光镜观察胸主动脉形态学变化;TUNEL染色观察胸主动脉内皮细胞凋亡情况;采用RT-PCR和免疫印迹法分别检测胸主动脉血管内皮细胞中钙敏感受体、Bax、Bcl-2及Caspase-3的mRNA和蛋白表达。结果与对照组比较,动脉粥样硬化组细胞凋亡指数以及钙敏感受体、Bax和Caspase-3的表达升高,Bcl-2的表达降低;光镜下可见胸主动脉部分内皮脱落,表面不光滑,中膜平滑肌细胞数目明显增多,排列紊乱。结论钙敏感受体可能参与了主动脉血管内皮细胞的凋亡,进而启动动脉粥样硬化的形成。

Aim To observe the expression of calcium sensing receptor（CaSR） in atherosclerosis rat vascular endothelial cells and the relationship with apoptosis. Methods 144 Wistar rats were randomly divided into control group（n=72） and atherosclerosis groups（n=72）. Early atherosclerosis models were copied by injection of vitamin D3 D360 million units/kg body weight,and then fed with high fat diet for 6 weeks. Serum triglyceride（TG） and total cholesterol（TC） were detected by automatic biochemistry analyzer. The morphological changes were observed under optical microscope. The mRNA and protein expressions of CaSR,Bcl-2,Bax and Caspase-3 were analyzed by Western blotting and RT-PCR. Apoptotic cells were measured by TUNEL staining. Results Compared with control group,the apoptosis index and the expression of CaSR,Bax and Caspase-3 were increased,Bcl-2 expression were decreased,the changes in aorta thoracica were aggravated in the atherosclerosis group. Conclusion CaSR may participate in the apoptosis of vascular endothelial cell,and then accelerate the formation of atherosclerosis.