枯草溶菌素转换酶9 siRNA抑制氧化型低密度脂蛋白诱导的THP-1源性巨噬细胞炎症因子表达

Proprotein Convertase Subtilisin/Kexin 9 siRNA Repressed Inflammation Factor Expression of THP-1 Macrophage Cells Induced by Oxidized Low Density Lipoprotein

目的观察枯草溶菌素转化酶9 siRNA对氧化型低密度脂蛋白诱导的THP-1源性巨噬细胞炎症因子表达的影响。方法应用Lipofectamine 2000分别转染80 nmol/L浓度枯草溶菌素转化酶9 siRNAs进THP-1源性巨噬细胞中,作用24 h后加入80 mg/L氧化型低密度脂蛋白处理24 h,采用逆转录聚合酶链反应检测白细胞介素1α、白细胞介素6和肿瘤坏死因子αmRNA的表达。结果 80 mg/L氧化型低密度脂蛋白刺激THP-1源性巨噬细胞24 h后,白细胞介素1α、白细胞介素6和肿瘤坏死因子-αmRNA的表达明显增加(P<0.05),而枯草溶菌素转化酶9 siRNA转染组THP-1源性巨噬细胞白细胞介素1α、白细胞介素6和肿瘤坏死因子αmRNA的表达相对于氧化型低密度脂蛋白组明显降低(P<0.05)。结论枯草溶菌素转化酶9 siRNA能够抑制氧化型低密度脂蛋白诱导的THP-1源性巨噬细胞炎症因子表达,枯草溶菌素转化酶9可能参与了炎症反应的调节。

Aim To investigate the effect of proprotein convertase subtilisin/kexin 9（PCSK9） siRNA on inflammatory factor expression in oxidized low density lipoprotein（ox-LDL） induced THP-1 macrophage cells. Methods The siRNAs for PCSK9 were designed and synthesized,then THP-1 macrophages were transfected with 80 nmol/L PCSK9 siRNA by positive ion liposome Lipofectamine 2000 for 24 h and then a high level of ox-LDL（80 mg/L） for an additional 24 h.Reverse transcription polymerase chain reaction（RT-PCR） was conducted to detect interleukin-1α（IL-1α） mRNA,interleukin-6（IL-6） mRNA and tumor necrosis factor-α（TNF-α） mRNA. Results Ox-LDL increased IL-1α mRNA,IL-6 mRNA and TNF-α mRNA expression.The expression of IL-1α mRNA,IL-6 mRNA and TNF-α mRNA in the macrophage pretreated with PCSK9 siRNA was decreased significantly,in comparison with ox-LDL-treated group（P0.05）. Conclusion PCSK9 siRNA can repress inflammation factor expression of THP-1 macrophage cells induced by ox-LDL.PCSK9 may be involved in adjustment for inflammation.