摘要
目的观察参松养心胶囊对压力超负荷所致心力衰竭大鼠的QT离散度及缝隙连接蛋白43表达的干预作用。方法采用腹主动脉缩窄法建立大鼠心力衰竭模型,经参松养心胶囊治疗8周。用皮下插入自制电极检测心室电生理变化,经HE染色观察其形态结构变化,Masson染色判断心肌纤维化沉积,免疫组织化学法观察缝隙连接蛋白43在左心室心肌组织的分布。结果心力衰竭大鼠QT离散度明显延长(37.20±9.94 ms,P<0.05),心肌细胞排列紊乱,心肌组织纤维沉积面积明显增加(101217.30±33970.02μm2,P<0.05),心肌组织缝隙连接蛋白43表达明显减少(55.93±11.61,P<0.05)。参松养心胶囊可缩短心力衰竭大鼠的QT离散度(25.50±8.21 ms),减少心肌组织纤维沉积面积(13580.64±8213.73μm2)并增加缝隙连接蛋白43的表达(69.09±16.59)。结论参松养心胶囊可以缩短心力衰竭大鼠的QT离散度,增加心肌组织缝隙连接蛋白43表达并减少纤维化面积。
Aim To investigate the effect of Shensongyangxin capsule on QT dispersion and connexin43 expression in heart failure rats. Methods Heart failure rats models were built by constricting abdominal aorta,and were lavaged with Shensongyangxin capsule for 8 weeks.Ventricular electrophysiology were measured by inserting home-made electrode into subcutaneous,left ventricular morphostructure,myocardial fibrosis,and connexin43 distribution were respectively observed by HE staining,Masson staining,immunohistochemical staining. Results Heart failure rats QT dispresion were significantly longer（37.20±9.94 ms,P0.05）,cardiomyocytes were misaligned,myocardial fibrosis area were significantly increased（101217.30±33970.02 μm2,P0.05）,and connexin43 distribution were significantly decreased（55.93±11.61,P0.05）.Shensongyangxin capsule can shorten QT dispresion（25.50±8.21 ms） of heart failure rats,increase connexin43 distribution（69.09±16.59） and decrease myocardial fibrosis area（13580.64±8213.73 μm2） in myocardium of heart failure rats. Conclusion Shensongyangxin capsule can shorten QT dispresion of heart failure rats,increase connexin43 distribution and decrease myocardial fibrosis area in myocardium of heart failure rats.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2011年第3期197-201,共5页
Chinese Journal of Arteriosclerosis
基金
中华医学会临床医学科研专项基金(09010280183)
