经下腔静脉逆灌注含氧自体稀释血对中低温停循环猪血清及肝肾MDA、SOD的影响

Effect of retrograde perfusion with autologous blood through inferior vena cava on MDA and SOD in serum,liver and kidney during moderate hypothermic lower body circulatory arrest in pigs

目的探讨中低温停循环时经下腔静脉逆行灌注对猪血清及肝、肾组织丙二醛(malondialdehyde,MDA)含量及超氧化物歧化酶(superoxide dismutase,SOD)活性的影响。方法选择健康小型猪20只,随机分为2组,每组10只。A组(对照组)给予中低温停循环90 min,B组(实验组)给予中低温停循环联合经下腔静脉逆行灌注90 min。检测逆灌注前(T1)、停循环1h(T2)及复灌1 h(T3)、3 h(T4)、5 h(T5)血清及肝、肾组织内MDA含量及SOD活性。结果血清及肝肾组织内MDA、SOD在T1时间点两组差异无统计学意义。在T3～T5时间点两组血清MDA含量均升高,SOD活性均下降,但B组变化幅度低于A组(P<0.05)。A组在T2～T5时间点肝、肾组织内MDA含量高于B组,而SOD活性低于B组(P<0.05)。结论经下腔静脉逆灌注含氧自体稀释血能部分改善中低温停循环猪肝、肾器官缺血再灌注损伤,对肝肾功能具有一定的保护作用。

Objective To study the effect of retrograde perfusion through the inferior vena cava（IVC） during moderate hypothermic lower body circulatory arrest on malondialdehyde（MDA） content and superoxide dismutase（SOD） activity in the serum,liver and kidney of pigs.Methods Twenty healthy pigs were randomly divided into two groups： group A had moderate hypothermic lower body circulatory arrest for 90 min,group B had moderate hypothermic lower body circulatory arrest and retrograde perfusion through IVC at the same time.SOD activities and MDA contents in the serum,liver and kidney were analyzed before retrograde perfusion（T1）,1 h after circulatory arrest（T2）,and 1 h（T3）,3 h（T4）,and 5 h（T5） after reperfusion.Results There were no significant differences in SOD activities and MDA contents in the serum,liver or kidney between the two groups at T1（P0.05）.Serum MDA contents were increased and the SOD activities were decreased in both groups at T3,T4,and T5,with the changes in group B being significantly small than those in group A（P0.05）.MDA contents in the liver and kidney in group A were significantly higher and the SOD activities were significantly lower than those in group B at T2-T5（P0.05）.Conclusion Retrograde perfusion with autologous blood through IVC can partly alleviate the ischemic-reperfusion injury of liver and kidney during moderate hypothermic lower body circulatory arrest in pigs,showing a protective effect on the hepatic and renal function.