载脂蛋白E和补体C4b1在人胰腺癌组织中的表达及意义

Apolipoprotein E and complement C4b1 expressions and its significance in human pancreatic carcinoma

目的 检测载脂蛋白E（ApoE）和补体C4b1在人胰腺癌组织中的表达,探讨其意义.方法 应用免疫组化法检测38例胰腺癌和相应癌旁正常胰腺组织ApoE、C4b1蛋白表达;应用RT-PCR法检测20例胰腺癌和相应癌旁正常胰腺组织ApoE、C4b1 mRNA表达.分析ApoE、C4b1的表达与胰腺癌生物特征的相关性.结果 胰腺癌组织ApoE、C4b1蛋白表达阳性率分别为86.8%（33/38）和76.3%（29/38）,显著高于癌旁正常胰腺组织的42.1%（16/38）和26.3%（10/38）（P值均＜0.01）;有淋巴结转移的胰腺癌组织ApoE、C4b1蛋白表达阳性率分别为78.3%（18/23）和73.9%（17/23）,明显高于无转移者的33.3%（5/15）和40.0%（6/15）（P值均＜0.05）.胰腺癌ApoE、C4b1 mRNA表达量分别为4.83±0.65、7.94±0.95,明显高于癌旁正常胰腺组织的1.78±0.74、1.22±0.57（P值均＜0.01）;有淋巴结转移的胰腺癌组织ApoE、C4b1 mRNA的表达量为5.05±0.71、8.24±1.07,显著高于无转移者的4.42±0.25、7.39±0.15（P值均＜0.05）.结论 ApoE、C4b1在胰腺癌组织中高表达,并与胰腺癌淋巴结转移相关.

Objective To investigate the expression of apolipoprotein E （ApoE） and complement C4b1 in pancreatic carcinoma and study its significance. Methods Immunohistochemistry was used to detect the expression of ApoE and C4b1 protein in 38 cases of pancreatic carcinoma tissues and adjacent normal pancreatic tissues, and RT-PCR was used to detect the expression of ApoE and C4b1 mRNA in 20 cases of pancreatic carcinoma tissues and adjancent normal pancreatic tissues. The relevance of ApoE and C4b1 expressions to the biological features of pancreatic carcinoma were analyzed. Results The positive rates of ApoE and C4b1 expressions are 86.8% （33/38） and 76.3% （29/38） in pancreatic carcinoma, respectively,which were significantly higher than those in normal pancreatic tissues [42.1% （16/38） and 26.3% （ 10/38 ）,P ＜ 0.01]. The positive rates of ApoE and C4b1 expressions [78.3% （ 18/23 ） and 73.9% （ 17/23 ）] in patients with metastasis were significantly higher than in those without metastasis [（33.3% （5/15） and 40.0%（6/15）, P ＜ 0.05）. Significantly higher expressions of ApoE and C4b1 mRNA were noted in pancreatic carcinoma（4.83 ± 0.65 and 7.94 ± 0. 95 ） than those in the normal pancreatic tissue （ 1.78 ± 0.74and 1.22 ±0.57, P ＜ 0.01 ）, and patients with metastasis showed significantly higher expression of ApoE and C4b1 mRNA （5.05 ±0.71 and 8.24 ± 1.07） than those without metastasis （4.42 ±0.25 and 7.39 ±0.15,P ＜ 0.05）. Conclusions ApoE and C4b1 were highly expressed in pancreatic cancer, and may be closely related with lymph node metastasis.