p53抑制剂PFT-α对阿霉素诱导的人皮肤角质形成细胞凋亡的影响

Effect of p53 Inhibitor-α on Adriamycin Induced Apoptosis of Cultured Human Skin Keratinocytes in Vitro

目的探讨p53抑制剂PFT-α对阿霉素诱导的人皮肤角质形成细胞凋亡的影响。方法用MTT法通过比色分析测定吸光度(OD)值,检测0,4,8,12mg/LPFT-α和阿霉素给药对角质形成细胞增殖的影响,用流式细胞仪检测细胞凋亡。结果阿霉素抑制体外培养的人皮肤角质形成细胞增殖,先给予8,12mg/LPFT-α处理细胞,然后给予阿霉素,与对照组比较,PFT-α升高OD值(P<0.05),降低细胞凋亡率(P<0.05)。结论先行给予适当剂量PFT-α处理人皮肤角质形成细胞,能一定程度减轻阿霉素对人皮肤角质形成细胞的损伤,表现出对阿霉素致凋亡的保护作用。

Objective To investigate the effect of p53 inhibitor-α （PFT-α） on adriamycin induced apoptosis of cultured human skin keratinocytes in vitro. Methods Human skin keratinocytes were cultured with 0, 4, 8, 12 mg/L PFT-α and adriamycin. Cell proliferation was detected by colorimetric assay of MTT. The optical density （OD） values were read on a scanning muhiwell spectrophotometer （ELISA reader）. Apoptosis rate of human skin keratinocytes was detected by flow cytometry. Results The proliferation of human skin keratinocytes was inhibited by adriamycin. If cells were pre-incubated with 8, 12mg/L PFT-α, OD value was significant higher and apoptosis induced by adriamycin was suppressed compared with control group （P 〈 0.05）. Conclusion This study suggested that PFT-α could partially inhibit adriamycin induced apoptosis of cultured human skin keratinocytes.