摘要
应用荧光原位杂交技术进行rDNA在黄花水仙和两色花水仙染色体上的定位研究,并分析了两色花水仙的基因组成。结果表明:不同材料的NOR位点表现出多态性,即黄花水仙上具3个45SrDNA位点,两色花水仙具有5个位点;5SrDNA在位点和数量上表现出一致性。基因组荧光原位杂交结果表明两色花水仙的染色体有10条来自于黄花水仙,22条来自于白花水仙。根据rDNA在这些种质资源上的分布,结合GISH技术的分析,证实两色花水仙是黄花水仙和白花水仙的天然杂交种。
Used florescence in situ hybridization（FISH）and genomic in situ hybridization（GISH）techniques to analyze the genomic organization in Narcissus tazetta var.chinensis Roem.distributed in China.Chromosome localization of 45S rDNA and 5S rDNA were studied by means of FISH on metaphase chromosomes of yellow flower narcissus and two-color flower narcissus.The results showed that NOR loci of different materials showed polymorphism.There were three 45S rDNA loci in yellow flower narcissus,while there were five loci in two-color flower narcissus.The distribution of 5S rDNA appeared similar in the two genomes.Based on GISH analysis,two-color flower narcissus（2n = 3x = 32）had two genomes of white flower narcissus（2n = 2x = 22）and a single genome of yellow flower narcissus（2n = 2x = 20）.By comparing the physical location of 45S rDNA and 5S rDNA on chromosomes and the result of GISH,it was believed that two-color flower narcissus was the result of nature hybridization between white flower narcissus and yellow flower narcissus.
出处
《园艺学报》
CAS
CSCD
北大核心
2011年第4期740-746,共7页
Acta Horticulturae Sinica
基金
国家科技支撑计划项目(2007BAD07B04)
福建省自然科学基金项目(2009J01066)
关键词
中国水仙
基因组原位杂交
荧光原位杂交
Narcissus tazetta var. chinensis Roem.
genomic in situ hybridization（GISH）
florescence in situ hybridization（FISH）
