阻塞性睡眠呼吸暂停低通气综合征软腭肌肌纤维亚型构成变化的研究

The configuration of soft palate muscular phenotype in obstructive sleep apnea hypopnea syndrome

目的:研究阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者相关的腭肌肌纤维亚型、胶原纤维mRNA表达情况及其病理形态学上的变化。方法:对12例OSAHS患者(实验组)及8例正常人(对照组)使用实时定量PCR方法检测其软腭肌各重链亚型肌纤维及胶原纤维mRNA的表达量。并利用肌肉ATP酶染色技术鉴别软腭肌3种肌纤维亚型Ⅰ型、ⅡA型与ⅡB型,计算各型肌纤维的数目。结果:实验组ⅡA亚型肌纤维mR-NA表达明显增多(P<0.01)。而Ⅰ型肌纤维在pH4.3染色条件下实验组数目低于对照组(P<0.05)。结论:与对照组比较,实验组收缩速度快、收缩力大但持久性差的ⅡA型肌纤维mRNA表达相对增多,ATP酶染色显示收缩速度慢、持久性好的Ⅰ型肌纤维细胞减少。

Objective：To study the mRNA expression of muscle phenotype and collagen of soft palate and pathology in obstructive sleep apnea hypopnea syndrome（OSAHS）.Method：We used the Real-time PCR to test the mRNA expression of soft palate muscle myosin heavy chain（MyHC） phenotype and collagen in 12 OSAHS patients and 8 control patients.We also distingushed the muscle isoforms Ⅰ,Ⅱ with ATPase staining,then counted the numbers of isoforms muscle fiber.Result：The mRNA expression of OSAHS group was more than control group in ⅡA MyHC phenotype（P0.01）.The number of OSAHS group muscle fibre Ⅰ isoform was less than control group with pH4.3 ATPase staining（P0.05）.Conclusion：Compare to control group,the enhancement happened in the mRNA expression of ⅡA MyHC phenotype which can increase the velocity and power but decrease the enduring quality of muscle in OSAHS,and the reducement be in the Ⅰ MyHC isoform of muscle fiber that can cause muscle velocity become slower and persistency become longer in OSAHS patients.