眼针对肠易激综合征模型大鼠结肠组织5-羟色胺重摄取转运体表达的影响

Effects of Oculo-acupuncture Therapy on Colonic Serotonin Reuptake Transporter Expression in Rats with Irritable Bowel Syndrome

目的:观察眼针对肠易激综合征(IBS)模型大鼠结肠组织5-羟色胺重摄取转运体(SERT)表达的影响,探讨眼针治疗IBS的作用机制。方法:雄性Wistar大鼠30只,随机分成对照组I、BS模型组、眼针组,每组10只。采用束缚刺激与应激刺激相结合的方法建立IBS动物模型,选取"下焦区""大肠区""肝区"及"脾区"行眼针干预,每12 h针刺1次,共针刺7 d。各组采用Western Blot法、RT-PCR法检测结肠组织中SERT的蛋白和mRNA表达。结果:与对照组比较,模型组SERT的mRNA和蛋白表达均下调(P<0.05);与模型组比较,眼针组SERT的mRNA和蛋白表达均上调(P<0.05)。结论:眼针干预可有效上调IBS模型大鼠SERT表达水平,进而对IBS起到治疗作用。

Objective To investigate the effect of oculo-acupuncture therapy （OAT） on the alteration of colonic serotonin reuptake transporter （SERT） expression in rats with irritable bowel syndrome （IBS） so as to explore its underlying mechanism in relieving IBS. Methods Thirty male Wistar rats were randomized into control, IBS model and OAT groups （ n = 10/group）. IBS model was established by repeated stress stimulation [water-intake inhibition （24 h）, warm box keeping （40 ℃ ,5 min）, elec-tric shock stimulation （30 V, 120 s） . forced cool water swimming （14 ℃, 5 min）, 24 h fasting, and tail clamping （180 s）] for 18 days. Qculo-acupuncture stimulation was given to ＂Xiajiao＂ （Lower Energizer）, ＂Dachang＂ （Large Intestine）. ＂Gan＂ （Liver） and ＂Pi＂ （Spleen） areas for 20 min by using filiform acupuncture needles （manipulated 1 min once every 10 min）. The treatment was conducted once every 12 h for 7 days. Colonic SERT mRNA and protein expression levels were examined by RT-PCR and Western Blot. Results In comparison with the control group, the expression levels of both colonic SERT mRNA and protein in the model group were down-regulated remarkably（ P〈0. 05）. Compared with the model group, the expression levels of SERT gene and protein in the OAT group were increased remarkably（ P〈0. 05）. Conclusion OAT can increase colonic SERT protein and gene expression in IBS rats. which may play a role in relieving IBS in OAT clinic.