水溶性氮酮对斑马鱼胚胎药物渗透及致畸性作用的影响

Effects of Azone on Osmotic Absorption of Resveratrol in Zebrafish

目的 研究水溶性氮酮对白藜芦醇透过斑马鱼胚胎性能与斑马鱼致畸性的影响.方法 利用对斑马鱼胚胎发育致畸作用,考察氮酮在斑马鱼筛选模型上的适用性,确定氮酮的使用剂量;利用氮酮的透皮促进作用,观察对白藜芦醇抑制斑马鱼节间血流的影响.结果 在0.01～0.5μg/ml范围内氮酮对斑马鱼较安全,0.5μg/ml溶性氮酮可显著促进斑马鱼对白藜芦醇的透过,斑马鱼节间血管的抑制率达到了22.9%.结论 浓度高于0.5 μg/ml的水溶性氮酮对斑马鱼胚胎的发育有明显致畸作用;0.5μg/ml的氮酮对白藜芦醇的斑马鱼胚胎透过效果最佳.

Objective To study the effects of water-solubility azone on the transdermal absorption of resveratrol and teratogenicity. Methods The formation of neovascularization was inhibited by using resveratrol, the number of zebrafish dorsal aorta intersegmental blood flow were calculated added with water-solubility azone under fluorescent microscope, and teratogenic-action effects were recorded by water-solubility azone added for 24 h. Results The water -solubility azone at the coneentration of 0.01-0.5 μg/ml were safe to zebrafish embry, and the 0.5μg/ml water-solubility azone showed significant transdermal absorption of resveratrol in zebrafish, intersegmental vessel number of dorsal aorta inhibition ratio could be 22.9%, the incidence of deformity can be occured. Conclusions The water-soluble azone with the Concentrations over 0.5 μg/ml have significant teratogenic effects on zebrafish embryos; and the 0.5μg/ml water-solubility azone shows the optimal transdermal absorption of the resveratrol.