转录激活因子5在直肠癌中的差异性表达

Differential Expression of Activating Transcription Factor 5 in Human Rectal Cancer

目的分析人直肠癌组织中转录激活因子5（ATF5）的表达变化及其与直肠癌临床病理特征的关系。方法选取四川大学华西医院胃肠外科中心2009年3～10月期间手术切除的直肠癌及距离肿瘤5cm以远的正常直肠组织标本92对，分别应用实时荧光定量RT-PCR和免疫组织化学染色SP法检测ATF5 mRNA和蛋白的表达情况。结果直肠癌组织中33例（35．9％）ATF5 mRNA表达上调，但ATF5 mRNA相对表达量与正常直肠组织相比，二者间差异无统计学意义（P=0．363），并且ATF5 mRNA表达与患者年龄、性别、肿瘤类型、分化程度、浸润深度、淋巴结转移、远处转移及TNM分期均无关（P〉0．05）；但直肠癌组织中ATF5蛋白的表达高于正常直肠组织（P=0．000），并且其表达与肿瘤分化程度有关（P=0．013），而与其他临床病理因素无关（P〉0．05）。结论ATF5蛋白的表达与直肠癌的发生和分化相关，但这种相关需要进一步的研究证明。

Objective To determine the expression change of activating transcription factor 5 （ATF5） in hu- man rectal cancer tissue, and analyze the correlation between ATF5 expression and the clinicopathologic parameters of rectal cancer. Methods Ninety-two paired samples of rectal cancer tissue and more than 5 cm distant normal rec- tal tissue were obtained from inpatients between March 2009 and October 2009 in this hospital. ATF5 mRNA and protein expressions were detected by quantitative real-time RT-PCR and immunohistoehemieal staining. Results Thirty-three （35.9 % ） cases of rectal cancer showed ATF5 mRNA overexpression~ however, the expression level of ATF5 mRNA in the rectal cancer tissue was not statistically different from that in the normal rectal tissue （P=0. 363）. There was no evidence for the relationship between the ATF5 mRNA expression and the patients＇ age, gender, histological type, tumor differentiation degree, invasive depth, lymph node metastasis, distance me- tastasis, or TNM stage. In contrast, the positive expression rate of ATF5 protein in the rectal cancer tissue was sig- nificantly higher than that in the normal rectal tissue （P：0. 000）. Moreover, the ATF5 protein expression was cor- related with the tumor differentiation degree （P：0. 013）, but not with other clinicopathologic features （P〉0.05）. Conclusions The results suggest that ATF5 protein may he related to the carcinogenesis and differentiation of hu- man rectal cancer. However, further researches are required to prove the correlation.