XIAP基因在慢性髓系白血病中的表达及临床意义

Expression of XIAP Gene in Chronic Myeloid Leukemia and Its Clinical Significance

本研究旨在检测慢性髓系白血病(CML)患者XIAP基因mRNA的表达情况,以探讨其在CML病情进展及预后中的意义。应用染色体R显带技术及实时PCR技术对71例CML患者和10名健康者进行染色体核型分析及XIAP mRNA检测。结果表明,XIAP mRNA在CML的加速期和急变期的平均表达水平明显高于慢性期(p<0.01)。XIAP基因在不同核型组的平均表达水平无显著性差异。结论:CML患者加速期和急变期XIAP基因表达明显增高,XIAP基因与CML的病情进展密切相关。

This study was purposed to investigate the expression of XIAP mRNA in chronic myeloid leukemia（CML） and to explore its significance in the advance and prognosis of CML.The chromosomal karyotype analysis and detection of XIAP mRNA were performed by the technique of chromosomal R banding and real time PCR in 71 patients with CML and 10 healthy controls.The results showed that there was a significant increase of XIAP mRNA expression in accelerated and blastic phase of the CML,compared with the patients in chronic phase（t=9.10,9.30,p〈0.01）.Moreover,the difference of XIAP mRNA expression level was not statistically significant in different karyotype groups.It is concluded that the XIAP gene expression in accelerated and blastic phases of CML patients obviously increases,XIAP gene is closely related to the advance of CML.