用组织块培养法从骨髓滤网颗粒物中分离大量骨髓间充质干细胞

Isolation of Mesenchymal Stem Cells from Bone Marrow Filters by Primary Explant Culture

本研究探讨能否从废弃的骨髓滤网颗粒物中获得间充质干细胞(MSC)。应用组织块培养法,对2例正常供者骨髓滤网中的颗粒物进行分离培养,计数获得的MSC数量,对其表型和分化能力进行鉴定。结果表明:骨髓滤网颗粒物中存在大量的MSC,经过3代扩增后其数量接近107;这些细胞具有典型的MSC形态和表型特征,可以分化为骨、软骨和脂肪细胞。结论:用组织块培养法可以从骨髓滤网颗粒物中获得大量MSC。

This study was aimed to investigate whether mesenchymal stem cells（MSC） can be isolated from bone marrow filters which have always been discarded.The bone marrow（BM） particles from BM filters of 2 healthy donors were cultivated by primary explant culture.After expansion,the number of MSC was counted and their immuno-phenotype and differentiation potential were detected.The results indicated that many MSC were found in bone marrow particles from filters,and nearly 107 MSC were obtained at 3 passages of expansion.They not only possessed the characteristics of morphology and immunophenotype of MSC,but also could differentiate into osteoblastes,chondrocytes and adipocytes.In conclusion,a large amount of MSC can be obtained from BM filters if the BM particles were cultivated by primary explant culture.