RECK在骨巨细胞瘤中的表达及与MMP-2的关系

Correlation between the protein expression of RECK and MMP-2 in giant cell tumor(GCT) of bone

目的检测RECK基因以及金属蛋白酶-2(MMP-2)在骨巨细胞瘤(GCT)以及骨软骨瘤中的表达情况,以探讨RECK基因的表达与GCT的发生、生长以及生物学行为之间的相关性。方法应用免疫组织化学SP方法检测30例GCT和10例骨软骨瘤肿瘤组织中RECK与MMP-2的表达,进而分析RECK、MMP-2在上述肿瘤组织中表达的阳性率及两者之间的相关性。结合临床资料,分析和比较RECK基因在不同外科学分期、影像学分级GCT肿瘤组织中表达的差异性,了解RECK基因的表达与GCT预后(复发率)之间有无关系。结果 RECK基因在GCT肿瘤组织中表达较对照组(骨软骨瘤)阳性率低(P<0.05),并且在不同外科学分期和影像学分级的GCT肿瘤组织,表达的阳性率差异有统计学意义(p<0.05)。同时发现RECK基因与MMP-2蛋白的表达呈负相关性。RECK基因的表达与GCT患者的性别、年龄、发病部位等无显著相关性,与肿瘤的预后(术后复发率)有相关性,但Logistic回归分析显示,RECK及MMP-2均不是GCT预后的独立危险因素,而手术方式是GCT预后的独立危险因素。结论 GCT肿瘤组织中RECK基因低表达、MMP-2高表达。RECK基因可能在骨巨细胞瘤的发生、生长过程中起重要作用。本研究结果可以为GCT的预后判断及分子靶向药物的开发提供实验依据。

Objective To investigate the relation between the expression of reversion-inducing-cysteinerich protein with kazal motifs （RECK） and the occurrence, growth and biological behavior of giant cell tumor （GCT） of bone through examing the expressions of RECK and matrix metallo proteinases 2 （MMP-2） in the GCT of bone and osteochondroma. Methods The immunohistochemical SP technique was applied to exam the expression of RECK and MMP-2 in tumor tissues of 30 cases with GCT and 10 cases with osteochondroma. And to analyze the positive rate of expressions of RECK and MMP-2 in GCT of bone and osteochondroma and the relation between the expression of RECK in GCT of bone and MMP-2 in osteochondroma. Clinical materials were integrated to compare and analyze the difference of RECK expression in tumor tissues of GCT in different Enneking surgical classification and Campanacci classification, and to understand whether any relation between the RECK expression and GCT prognosis （recurrence rate） exists. Results Compared with control group with osteochondroma, the expressions of RECK in GCT was of lower positive rate （P〈0.05）. Significant deference of positive rate existed in RECK expression of tumor tissues in GCT in different Enneking surgical classification and Campanacci classification （P〈0.05）. Meanwhile, it was found that RECK and the expression of MMP-2 were inversely correlated. No significant correlation between the expression of RECK and the sex, age and invasive sites of patients with GCT. Correlation existed between the expression of RECK and the prognosis of tumor （recurrence rate after the surgery）. But logistic regression analysis showed that both RECK and MMP-2 were not the independent risk factors of GCT prognosis, while the procedure of surgery was the independent risk factors of GCT prognosis. Conclusions RECK is less expressed while MMP-2 is more expressed in GCT. RECK may play an important part in the invasion and growth of GCT of bone. The research results may provide experimental basis for the prognosis of GCT and the molecular targeted therapeutic drugs development.