金芪降糖片对糖尿病大鼠血清基质金属蛋白酶9水平和外周血单核细胞基质金属蛋白酶9表达的影响

Effects of Chinese herbal medicine Jinqi Jiangtang Tablet on serum matrix metalloproteinase-9 level and its expression in peripheral blood monocytes of rats with diabetes mellitus

目的:观察中药金芪降糖片对链脲佐菌素诱导的糖尿病大鼠血清基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)水平和外周血单核细胞MMP-9表达的影响。方法:30只Wistar大鼠随机分为3组:对照组、模型组及金芪降糖片组,每组10只。模型组与金芪降糖片组大鼠腹腔注射链脲佐菌素制作糖尿病大鼠模型后,金芪降糖片组予金芪降糖片溶液[0.8g/(kg.d)]灌胃6周,对照组和模型组以等体积生理盐水灌胃。灌胃6周后,下腔静脉取血分离血清,酶联免疫吸附测定法检测血清MMP-9水平;分离大鼠外周血单核细胞,采用逆转录聚合酶链反应和Western blot方法分别检测MMP-9mRNA及蛋白的表达。结果:治疗后,金芪降糖片组大鼠空腹血糖含量与模型组比较明显下降(P<0.05);建模成功后与对照组比较,模型组及金芪降糖片组大鼠血清MMP-9水平升高(P<0.01);灌胃6周后,与模型组比较,金芪降糖片组大鼠血清MMP-9水平降低(P<0.05);与对照组比较,模型组大鼠外周血单核细胞MMP-9表达增加(P<0.05);金芪降糖片组大鼠外周血单核细胞MMP-9表达较模型组降低(P<0.05)。结论:金芪降糖片可以降低糖尿病大鼠血清MMP-9水平,下调外周血单核细胞中MMP-9的表达。

Objective：To observe the effects of Jinqi Jiangtang Tablet,a compound Chinese herbal medicine,on serum matrix metalloproteinase-9（MMP-9） level and MMP-9 expression in peripheral blood monocytes of rats with streptozocin-induced diabetes.Methods：Thirty Wistar rats were randomly divided into 3 groups：control group,untreated group and Jinqi Jiangtang Tablet group.Diabetes in rats was induced by a single intraperitoneal injection of streptozocin（70 mg/kg body weight）.After model building,rats in the Jinqi Jiangtang Tablet group were administered with Jinqi Jiangtang tablets at a dose of 0.8 g/（kgd） for 6 weeks and rats in the control group and the untreated group were treated with normal saline.After treatment,level of fasting serum glucose was detected by hexokinas method;serum MMP-9 level was tested by enzyme-linked immunosorbent assay;MMP-9 expression in peripheral blood monocytes was measured by reverse transcription-polymerase chain reaction and Western blot method.Results：After treatment,fasting serum glucose level in the Jinqi Jiangtang Tablet group was less than that in the untreated group（P0.05）.After modeling,the serum MMP-9 level of rats in the untreated group was（9.11±3.55） μg/L and that in Jinqi Jiangtang Tablet group was（8.97±3.07） μg/L,which were higher than that in the control group（（4.39±1.44） μg/L）（P0.01）.After gavage,the level of serum MMP-9 was lower in Jinqi Jiangtang Tablet group than in the untreated group（P0.05）.The expression of MMP-9 in peripheral blood monocytes in the untreated group was higher than that in the control group（P0.05）.The MMP-9 expression in peripheral blood monocytes of rats with diabetes was down-regulated by Jinqi Jiangtang Tablet（P0.05）.Conclusion：Jinqi Jiangtang Tablet can decrease the level of serum MMP-9 and down-regulate the MMP-9 expression in peripheral blood monocytes at both mRNA and protein levels in rats with diabetes.