蜂蜜对炎症模型大鼠的抗炎作用及对一氧化氮生成的影响(英文)

Effects of honey on inflammation and nitric oxide production in Wistar rats

目的:研究蜂蜜对大鼠急慢性炎症及一氧化氮生成的作用。方法:使用角叉菜胶法、棉球法及甲醛致炎症法定量测定蜂蜜的抗炎症功效。硝基左旋精氨酸甲酯皮下注射(100mg/kg)及左旋精氨酸腹腔内注射(300mg/kg)检验蜂蜜对于大鼠一氧化氮生成的作用。在每个实验中,大鼠均被分为5组,每组5只大鼠。空白对照组及阳性对照组大鼠分别给予蒸馏水及消炎痛(5mg/kg)口服,其余3组大鼠分别给予2、6及10g/kg蜂蜜口服。结果:在角叉菜胶所致大鼠炎症模型实验中,与对照组比较,蜂蜜显著降低了大鼠后爪的肿胀程度(P<0.05);在棉球致大鼠肉芽肿瘤模型实验中,与对照组比较,蜂蜜显著降低了肉芽肿瘤的重量(P<0.05);在甲醛所致大鼠炎症模型实验中,与对照组比较,蜂蜜显著缓解了大鼠关节炎的症状(P<0.05)。在检测一氧化氮生成的实验中,硝基左旋精氨酸甲酯显著抑制了大鼠后爪的肿胀,而左旋精氨酸拮抗了蜂蜜及硝基左旋精氨酸甲酯的抗炎作用。结论:本实验的研究结果证明了蜂蜜的抗炎症作用,这可能与其抑制一氧化氮的生成有关。据此,蜂蜜可能可以被应用于某些急慢性炎症的治疗当中。

Objective：The aim of the study is to investigate the effects of honey on acute and chronic inflammations and nitric oxide production in rats.Methods：Carrageenan,cotton pellet and formaldehyde methods were used in quantifying the anti-inflammatory effect of honey while the effect of honey on nitric oxide（NO） production was investigated by administering NG-nitro-L-arginine methyl ester（L-NAME,100 mg/kg body weight,subcutaneously） and L-arginine（300 mg/kg body weight,intraperitoneally） to groups of rats.Animals were divided into five groups each comprising of five rats in each experiment;two groups were orally administered distilled water（control） and indomethacin（5 mg/kg body weight）,respectively,while the remaining three groups were administered 2,6 and 10 g/kg honey for anti-inflammatory studies.Results：Honey significantly（P0.05） reduced the paw size in the carrageenan model,while in the cotton pellet model,the granuloma weight was significantly（P0.05） reduced.Honey also significantly（P0.05） reduced the arthritis induced by formaldehyde injection from the second day of the study.In the investigation on NO involvement,L-NAME significantly inhibited paw oedema while the administration of L-arginine abolished the anti-inflammatory effect of honey and L-NAME.Conclusion：The results obtained from the study confirm that honey has an anti-inflammatory effect which may be due in part to inhibition of NO release.Therefore honey may be used to treat certain acute and chronic inflammatory conditions.