亚急性创伤性深静脉血栓形成模型大鼠基质金属蛋白酶3的表达

Expression of matrix metalloproteinase-3 in a rat model of subtraumatic deep vein thrombosis

背景:创伤性深静脉血栓发生率和死亡率呈逐年递增趋势,但其发生发展机制尚未清楚。因此有必要采用先进的基因芯片技术对其进行研究,探索其发生发展的内在规律。目的:观察创伤性深静脉血栓形成过程中基质金属蛋白酶3的表达变化规律,探讨其在创伤性深静脉血栓形成中的作用机制。方法:从110只SD大鼠中随机取10只大鼠作为正常组,其余100只大鼠作为实验组,采用定量击打双侧大腿+髋人字石膏固定方式,建立大鼠亚急性创伤性深静脉血栓动物模型。在伤后即刻、24,72,120和168h时相点切取股静脉血管组织,随后抽取总RNA,采用Affymetrix 2302.0芯片对股静脉血管组织进行基因表达检测。结果与结论:在亚急性创伤性深静脉血栓形成演化过程中,基质金属蛋白酶3在实验组的表达随伤后时间的延长而增加,说明其参与血栓形成过程,可能是影响深静脉血栓生物学状态的主要因素之一。

BACKGROUND： Incidence and mortality of traumatic deep vein thrombosis （TDVT） gradually increased in recent years, which generation mechanism remains unclear. Accordingly, it is necessary to explore inherent rules using gene chip technology. OBJECTIVE： To explore the changes and rules of matrix metalloproteinases in the TDVT. METHODS： Ten SD rats selected from 110 served as normal controls, other 100 rats were prepared subtraumatic TDVT models by beating on bilateral posterior limbs combined with hip spica cast fixation. The femoral vein was incised at immediately, 24, 72, 120 and 168 hours after operation. Total RNA was extracted from femoral vein, and the gene expression was detected by Affymetrix 230 2.0 genechips. RESULTS AND CONCLUSION： Expression of matrix metalloproteinase-3 was increased with prolongation of damage duration in the experimental group, suggesting matrix metalloproteinase-3 can be one of the most major factors to influence the biological states of TDVT.