光动力学照射小鼠牙周组织的病理变化

Pathological changes of mouse periodontal tissues following photodynamic therapy

背景:光化学疗法对口腔组织可能具有伤害,在临床应用甲苯胺蓝O介导的光动力学疗法预防龋病前,需进行生物安全性评估。目的:观察甲苯胺蓝介导的光动力学疗法产生的光敏作用对小鼠牙周组织健康的影响。方法:将54只小鼠随机分为9组,分别用0,50,100mg/L甲苯胺蓝O联合二极管光照0,15,25min。作用后72h,切取牙周组织切片观察。结果与结论:经光照治疗后小鼠的牙龈、牙槽骨和牙齿没有发现有坏死、溃疡、增生等异常改变。提示甲苯胺蓝介导的光动力学疗法一个安全的预防龋病的方法,对牙齿临近的牙周组织无破坏性影响。

BACKGROUND： Photodynamic therapy （PDT） may have bad effects on oral cavity tissue, so the biological safety evaluation is necessary before Toluidine blue O （TBO）-mediated PDT of dentine caries in clinic. OBJECTIVE： To observe the effects of photosensitization of the TBO-mediated PDT to periodontal tissues in mice. METHODS： Fifty-four mice were randomly divided into nine groups, and were respectively treated with 0 mg/L, 50 mg/L, 100 mg/L TBO with 0, 15, 25min LED illumination. Periodontal tissue slices were taken to observation after treatment of 72 hours. RESULTS AND CONCLUSION： There were no abnormal changes like necrosis, ulcer or hyperplasia happens in the mice gingival, alveolar bone or teeth after light treatment. This indicated that TBO-mediated PDT was safe to the prevention of dental caries, without damaging effect to periodonta~ tissues adjacent to the teeth.