氯化胆碱与制动性骨骼肌萎缩肌肉生成抑制素mRNA的表达

Effects of choline chloride on myostatin mRNA expression in rats with immobilization-induced skeletal muscle atrophy

背景:研究发现类胆碱物质可增加乙酰胆碱的弥散及终板电流的幅度,对神经肌肉接点功能退化有一定的对抗作用。目的:观察氯化胆碱对制动性骨骼肌萎缩的防治作用及对骨骼肌萎缩大鼠肌肉生成抑制素mRNA表达的影响。方法:将30只雄性SD大鼠随机分为对照组、模型组和治疗组,每组10只。采用可塑性石膏固定模型组和治疗组大鼠右后肢制备肌萎缩模型。治疗组每日灌胃氯化胆碱(150mg/kg),对照组和模型组灌胃等体积蒸馏水。4周后解剖右后肢腓肠肌,检测腓肠肌收缩张力、肌湿质量、蛋白质水平及肌肉生成抑制素mRNA的表达。结果与结论:与对照组比较,模型组大鼠腓肠肌的收缩张力、肌湿质量、蛋白质水平均显著降低(P<0.05或P<0.01),肌肉生成抑制素mRNA表达显著增高(P<0.01)。与模型组比较,治疗组大鼠腓肠肌的收缩张力、肌湿质量、蛋白质水平均显著升高(P<0.05),肌肉生成抑制素mRNA表达显著降低(P<0.05)。说明氯化胆碱能够显著提高制动性萎缩骨骼肌的收缩张力、肌湿质量、蛋白质水平,减少肌肉生成抑制素mRNA的表达,从而有效抑制骨骼肌制动性萎缩的发生。

BACKGROUND： Previous studies demonstrated that choline-based material can not only increase dispersion of acetylcholine and amplitude of endplate current, but also play an antagonistic action on function reduction of neuromuscular junction. I OBJECTIVE： To observe the prevention effects of choline chloride on immobilization-induced skeletal muscle atrophy and myostatin mRNA expression.METHODS： Thirty male SD rats were divided into control group, model group and treatment group, with 10 rats in each group. The right hind limbs of the rats were fixed with compliant plaster to prepare atrophy models. The rats in the treatment group received choline chloride （150mg/kg）. The same volume of distilled water was intragastric administrated into rats of the control and model groups. After 4 weeks, calf muscle in right hind limb were dissected and measured for the contractility, wet weight, protein content, and myostatin mRNA expression. RESULTS AND CONCLUSION： Compared with the control group, the contractility, wet weight, and protein levels in the calf muscle of the model group were dramatically decreased （P〈0.05 or P〈0.01）, but the myostatin mRNA expression was obviously increased （P〈0.01）. Compared with the model group, the contractility, wet weight, and protein levels in the calf muscle of thetreatment group were dramatically increased （P〈O.O5）,Gndthe the myostyaiin mRna expression was obviously decreased（P〈0.01）,The findings demonstrated that, choline chloride can improve contractility, wet weight, and protein levels in the calf muscles, and decrease myostatin mRNA expression, accordingly, choline chloride has preventive and protective effects on development of d suse atrophy of ske etal muscles.