透骨消痛颗粒干预膝骨性关节炎早期环氧合酶2和诱导型一氧化氮合酶的表达

Expression of cyclooxygenase-2 and inducible nitric oxide synthase in primary knee osteoarthritis interfered by Tougu Xiaotong granules

背景:由巴戟天、杭白芍、肿节风、川芎等组成的透骨消痛颗粒能有效改善膝骨性关节炎的症状,但其具体机制尚不清楚。目的:观察透骨消痛颗粒对膝骨性关节炎早期环氧合酶2和诱导型一氧化氮合酶表达的影响。方法:将90只新西兰大白兔随机等分为6组:除正常组外均采用改良Hulth法建立骨性关节炎动物模型,透骨消痛颗粒低、中、高剂量组及壮骨关节丸组分别在造模的基础上每天灌胃给予含透骨消痛颗粒5,10,20g或壮骨关节丸10g的水溶液。4周后,检测兔关节滑液中一氧化氮、一氧化氮合酶、前列腺素E2及关节软骨和滑膜组织中诱导型一氧化氮合酶、环氧合酶2的水平。结果与结论:骨性关节炎早期,关节滑液中一氧化氮、一氧化氮合酶、前列腺素E2水平及滑膜和软骨中环氧合酶2、诱导型一氧化氮合酶表达均明显升高。透骨消痛颗粒及壮骨关节丸均可降低关节滑液中一氧化氮、一氧化氮合酶、前列腺素E2及滑膜和软骨中环氧合酶2、诱导型一氧化氮合酶的表达,以透骨消痛颗粒中、高剂量组作用更明显。提示透骨消痛颗粒可通过抑制环氧合酶2和诱导型一氧化氮合酶表达,从而抑制软骨降解,缓解临床症状,达到保护关节软骨、防治骨性关节炎的作用。

BACKGROUND： Mornda root, radix paeoniae alba from Hangzhou of China, zhongjiefeng, szechwan Iovage rhizome composed of Tougu Xiaotong granule can effectively improve the symptoms of knee osteoarthritis （KOA）, but the mechanism of it remains unclear. OBJECTIVE： To observe the effect of Tougu Xiaotong granule on primary knee osteoarthritis cyclooxygenase-2 （COX-2） and inducible nitric oxide synthase （iNOS） expression. METHODS： A total of 90 New Zealand White rabbits were randomly divided into 6 groups： normal group, model group, Tougu Xiaotong granule low-dosage group, Tougu Xiaotong granule midst-dose group, Tougu Xiaotong granule high-dose group, and 7huangguguanjie pill group. In addition to the normal group, osteoarthritis model was established by improved Hulth method in the other groups. Tougu Xiaotong granule low-dosage group, Tougu Xiaotong granule midst-dose group, Tougu Xiaotong granule high-dose group, and Zhuangguguanjie pill group was based on modeling treated with containing 5, 10, 20 g TouguXiaotong granule or Zhuangguguanjie aqueous solution of 10 g pill at every day, respectively. After 4 weeks, the level of nitrogen monoxidum （NO）, NOS, and prostaglandin E2 （PGE2） in rabbit synovial fluid, articular cartilage, iNOS and COX-2 in synovium tissue was detected. RESULTS AND CONCLUSION： In the early stage of osteoarthritis, the level of NO, NOS, PGE2, and the expression of COX-2, iNOS in synovium and cartilage were higher than that in normal group. Tougu Xiaotong granule and Zhuangguguanjie pill can reduce the expression of NO, NOS, PGE2, and expression of COX-2 and iNOS in synovium and cartilage. Tougu Xiaetong granule midst-dose group and high-dose group have the most significant effects, it is indicated that Tougu Xiaotong granule can inhibit degradation of cartilage, release clinical symptoms to protect articular cartilage, prevent and cure osteoarthritis by COX-2 and iNOS expression.